A fast clean-up method has been developed to purify maize extracts and to detect moniliformin (MON) in maize samples from North-West Italy over a four-year period (2008-2011). The method is based on the use of MycoSep® 240 Mon clean-up columns (Romer Labs®). Samples were extracted using acetonitrile/water (84:16, v/v), and the extracts were purified in the previously described clean-up columns. The LC-MS/MS analysis has been carried out by means of hydrophilic interaction chromatography (HILIC), combined with negative electrospray mass spectrometry (ESI- - MS). The developed method has a recovery rate of 76-91% (RSD%: 6-14%), a limit of detection (LOD) of 1 µg kg-1, and a limit of quantification (LOQ) of 4 µg kg-1. One hundred and eight different naturally contaminated maize samples were analyzed for their MON content. The average percentages of positive samples was 93% with the following ranges (µg kg-1): 33-2606 (2008); < LOD-527 (2009); < LOD-920 (2010); < LOD-409 (2011).

Moniliformin Analysis in Maize Samples from North-West Italy Using Multifunctional Clean-up Columns and the LC-MS/MS Detection Method.

SCARPINO, VALENTINA;BLANDINO, Massimo;VANARA, Francesca;NEGRE, Michèle;REYNERI, Amedeo
2013-01-01

Abstract

A fast clean-up method has been developed to purify maize extracts and to detect moniliformin (MON) in maize samples from North-West Italy over a four-year period (2008-2011). The method is based on the use of MycoSep® 240 Mon clean-up columns (Romer Labs®). Samples were extracted using acetonitrile/water (84:16, v/v), and the extracts were purified in the previously described clean-up columns. The LC-MS/MS analysis has been carried out by means of hydrophilic interaction chromatography (HILIC), combined with negative electrospray mass spectrometry (ESI- - MS). The developed method has a recovery rate of 76-91% (RSD%: 6-14%), a limit of detection (LOD) of 1 µg kg-1, and a limit of quantification (LOQ) of 4 µg kg-1. One hundred and eight different naturally contaminated maize samples were analyzed for their MON content. The average percentages of positive samples was 93% with the following ranges (µg kg-1): 33-2606 (2008); < LOD-527 (2009); < LOD-920 (2010); < LOD-409 (2011).
2013
5
30
876
884
Moniliformin; Maize; LC-MS/MS; mycosep clean-up columns
SCARPINO V.; BLANDINO M.; VANARA F.; NEGRE M.; REYNERI A.
File in questo prodotto:
File Dimensione Formato  
Scarpino et al. 2013 FA&C.pdf

Open Access dal 01/08/2015

Descrizione: Post Print
Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 276.15 kB
Formato Adobe PDF
276.15 kB Adobe PDF Visualizza/Apri
TFAC_2013.pdf

Accesso riservato

Descrizione: Pdf editoriale accesso riservato
Tipo di file: PDF EDITORIALE
Dimensione 253.76 kB
Formato Adobe PDF
253.76 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/136210
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 24
  • ???jsp.display-item.citation.isi??? 18
social impact