The Influence of Electrospun Gelatin Fiber Diameter on Schwann cell adhesion and proliferation

For nerve tissue engineering application, the distribution and growth of cells on a scaffold are key requirements. Basal lamina membrane in different tissue has a distinctive nanofibrous features. Additionally, a growing number of studies demonstrates that micro-to-nanoscale topography plays an important role in controlling cell adhesion, proliferation and survival. Gelatin (GL) scaffold material may be promising in peripheral nerve tissue engineering due to its biocompatibility and biodegradability. Electrospinng is a simple and versatile technique for the production of random or align micro or nanofibers and it has successfully applied to different types of natural and synthetic polymers. In this study, gelatin micro or nanofibers matrices were prepared by electrospinning technique by varying the GL concentration and the flow rate. In order to study the influence of fibers diameter on cell adhesion and proliferation, in vitro test were performed by using Schwann cell (SC) since they have an important role in peripheral nerve regeneration.