In this study, we investigated the population of microRNAs present in leaf and root tissues of Cabernet Sauvignon (CS) and M4 rootstock grapevine genotypes under water and salt stress. Physiological parameters were daily measured on stressed and irrigated plants, in order to set the exact day of sampling (gs < 0.05 mmol H2O m-2 s-1 and Ψleaf ~ -1.4 MPa). Low Molecular Weight RNA (LMW RNA) was extracted and used to prepare cDNA libraries, which are in process of being sequenced by means of SOLiD platform and which were used for q-PCR analysis. The expression of several conserved miRNAs was analyzed by qRT-PCR using TaqMan probes. In several cases, expression showed differences between leaves and roots. Some miRNAs (such as e.g. miR159) were overexpressed in both leaves and roots of stressed CS and M4 plants, while the expression of others (miR393) was exclusively activated in leaves. A few miRNAs were differentially expressed between CS and rootstock genotype. Further analyses are underway on grafted plants, in order to gain evidence on possible miRNA transport between scion and rootstock.
Effects of stress on miRNA abundance in grapevine
PAGLIARANI, CHIARA;CARDINALE, Francesca;SCHUBERT, Andrea
2012-01-01
Abstract
In this study, we investigated the population of microRNAs present in leaf and root tissues of Cabernet Sauvignon (CS) and M4 rootstock grapevine genotypes under water and salt stress. Physiological parameters were daily measured on stressed and irrigated plants, in order to set the exact day of sampling (gs < 0.05 mmol H2O m-2 s-1 and Ψleaf ~ -1.4 MPa). Low Molecular Weight RNA (LMW RNA) was extracted and used to prepare cDNA libraries, which are in process of being sequenced by means of SOLiD platform and which were used for q-PCR analysis. The expression of several conserved miRNAs was analyzed by qRT-PCR using TaqMan probes. In several cases, expression showed differences between leaves and roots. Some miRNAs (such as e.g. miR159) were overexpressed in both leaves and roots of stressed CS and M4 plants, while the expression of others (miR393) was exclusively activated in leaves. A few miRNAs were differentially expressed between CS and rootstock genotype. Further analyses are underway on grafted plants, in order to gain evidence on possible miRNA transport between scion and rootstock.
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