Introduction: A protective role against atherosclerosis and cardiovascular disease has been attributed to Mediterranean dietary components, which include carotenoids. Among these lycopene, present in tomatoes and tomato products, has probably the highest antioxidant capacity but contrasting data have been reported about bioavailability and efficacy in humans. The aim of the study was to evaluate the effects of lycopene on oxidative stress in children affected by primary dyslipidemia. Material and methods: Twenty-height dyslipidemic children (7 Familial Hypercholesterolemia, 8 Familial Combined Hyperlipidemia, 13 Autosomal Dominant Hypercholesterolemia), aged 10.7 ± 2.4 ys, were enrolled in a double-blind, randomized, placebo-controlled, cross-over trial. Children received a dietary supplement containing lycopene (7 mg) or placebo for 4 weeks, separated by a 4-week washout period. Lipid profile (TC, HDL-C, TG, ApoB and ApoA-I) was assessed at baseline and after each treatment period by automatic analyzer (Olympus AU 2700, Japan). The concentration of OxLDL in plasma samples stored at -80°C were measured by an ELISA procedure using a commercial kit (Biomedica Gruppe). Urine samples were collected and stored at -80°C and assayed for quantification of the 8-iso-PGF2α-Isoprostane Enzyme Immunoassay (Cayman Chemical). Results: Baseline lipid profile examination showed: TC 230.4±34.7 mg/dl, HDL-C 55.6± 15.1 mg/dl, TG 76(38-171), LDL-C 157.8±31 mg/dl, ApoB 111.5±18 mg/dl, ApoA-I 142.1±24.4 mg/dl. Lycopene supplementation did not determine any variation of lipid profile, compared to placebo. Baseline OxLDL did not differ according to sex, age and diagnosis OxLDL levels resulted 633 (172-2524) ng/mL at baseline, 953 (506-3432) ng/mL and 938 (262-3260) ng/mL after lycopene and placebo treatment respectively. Data analysis concerning urinary 8-iso-PGF2α-Isoprostane are in progress. Conclusions: Lycopene supplementation did not show any significant effect on lipid profile and OxLDL in children affected by primary dyslipidemia.
Effects of lycopene supplementation on oxidative stess in children affected by primary dyslipidemia.
ABELLO, Francesca;Cena C.;GIORGIS, Marta;GUARDAMAGNA, Ornella
2011-01-01
Abstract
Introduction: A protective role against atherosclerosis and cardiovascular disease has been attributed to Mediterranean dietary components, which include carotenoids. Among these lycopene, present in tomatoes and tomato products, has probably the highest antioxidant capacity but contrasting data have been reported about bioavailability and efficacy in humans. The aim of the study was to evaluate the effects of lycopene on oxidative stress in children affected by primary dyslipidemia. Material and methods: Twenty-height dyslipidemic children (7 Familial Hypercholesterolemia, 8 Familial Combined Hyperlipidemia, 13 Autosomal Dominant Hypercholesterolemia), aged 10.7 ± 2.4 ys, were enrolled in a double-blind, randomized, placebo-controlled, cross-over trial. Children received a dietary supplement containing lycopene (7 mg) or placebo for 4 weeks, separated by a 4-week washout period. Lipid profile (TC, HDL-C, TG, ApoB and ApoA-I) was assessed at baseline and after each treatment period by automatic analyzer (Olympus AU 2700, Japan). The concentration of OxLDL in plasma samples stored at -80°C were measured by an ELISA procedure using a commercial kit (Biomedica Gruppe). Urine samples were collected and stored at -80°C and assayed for quantification of the 8-iso-PGF2α-Isoprostane Enzyme Immunoassay (Cayman Chemical). Results: Baseline lipid profile examination showed: TC 230.4±34.7 mg/dl, HDL-C 55.6± 15.1 mg/dl, TG 76(38-171), LDL-C 157.8±31 mg/dl, ApoB 111.5±18 mg/dl, ApoA-I 142.1±24.4 mg/dl. Lycopene supplementation did not determine any variation of lipid profile, compared to placebo. Baseline OxLDL did not differ according to sex, age and diagnosis OxLDL levels resulted 633 (172-2524) ng/mL at baseline, 953 (506-3432) ng/mL and 938 (262-3260) ng/mL after lycopene and placebo treatment respectively. Data analysis concerning urinary 8-iso-PGF2α-Isoprostane are in progress. Conclusions: Lycopene supplementation did not show any significant effect on lipid profile and OxLDL in children affected by primary dyslipidemia.
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