In the last decades lipids became the key players of study about human diseases because of their fundamental role in a broad range of biochemical processes, such as calcium homeostasis and membrane trafficking [1]. The lipidomic is the “omic” discipline that provide a comprehensive description of all cellular lipids (untargeted approach) and that characterize these latter in various physiological and pathological conditions (targeted approach) [2]. In this study we developed two methods to characterize the triglycerides and fatty acids profiles in mice treated with different high-fat contents diets. The first one was used to quantify the amount of triglycerides in several biological matrices (liver, and muscle tissues) from the mice, and it is based on liquid chromatography coupled with mass spectrometry [3]. The separation was performed on a RP C18 column with acetonitrile and dichloromethane with an 80 minutes long run gradient. The LTQ-Orbitrap was equipped with an APCI source and nitrogen was used as carrier gas. The second method was a gas chromatography-mass spectrometry method developed to quantify the methyl ester fatty acids profile in the biological matrices from the same mice. The correlation between triglycerides and fatty acids profile together with the different high-fat contents diets and biological matrices, and finally with the pathologies of mice (high glucose blood levels and insulin-dependent pathologies) was studied.

A LIPIDOMIC STUDY OF HIGH-FAT DIET TREATED MICE.

DAL BELLO, FEDERICA;SANTORO, VALENTINA;ARAGNO, Manuela;MASTROCOLA, Raffaella;MEDANA, Claudio;BAIOCCHI, Claudio
2014

Abstract

In the last decades lipids became the key players of study about human diseases because of their fundamental role in a broad range of biochemical processes, such as calcium homeostasis and membrane trafficking [1]. The lipidomic is the “omic” discipline that provide a comprehensive description of all cellular lipids (untargeted approach) and that characterize these latter in various physiological and pathological conditions (targeted approach) [2]. In this study we developed two methods to characterize the triglycerides and fatty acids profiles in mice treated with different high-fat contents diets. The first one was used to quantify the amount of triglycerides in several biological matrices (liver, and muscle tissues) from the mice, and it is based on liquid chromatography coupled with mass spectrometry [3]. The separation was performed on a RP C18 column with acetonitrile and dichloromethane with an 80 minutes long run gradient. The LTQ-Orbitrap was equipped with an APCI source and nitrogen was used as carrier gas. The second method was a gas chromatography-mass spectrometry method developed to quantify the methyl ester fatty acids profile in the biological matrices from the same mice. The correlation between triglycerides and fatty acids profile together with the different high-fat contents diets and biological matrices, and finally with the pathologies of mice (high glucose blood levels and insulin-dependent pathologies) was studied.
La spettrometria di massa in Piemonte
Torino
10/10/2014
La spettrometria di massa in Piemonte
33
33
http://piemonte.spettrometriadimassa.it/
Federica Dal Bello; Valentina Santoro; Manuela Aragno; Raffaella Mastrocola; Claudio Medana; Claudio Baiocchi.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/153711
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact