Betaine is an amino acid widely spread in animals and plants, such as beetroots. They accumulate polar betaine in response to water stresses. It takes part in the protection of cells, proteins and enzymes from environmental stress. Moreover betaine participates in the methionine cycle in the human liver and kidneys. The most important is however its biological importance in reducing cancer development. Despite these biological effects of betaine, there are just few methods for its extraction from beetroots, such as e.g. solid phase extraction. However quantitative recoveries were not achieved in these procedures. The main aim of present investigation was the development of a new, quick and effective procedure for the isolation and determination of betaine from two different varieties of Beta Vulgaris (Red and Gold). For the first time an accelerated solvent extraction (ASE) and solid phase extraction (SPE) were used to isolate betaine from interfering species and clean plant extracts. Additionally, a micro extraction by packed sorbent and a modified QuEChERS procedure were also tested. Qualitative and quantitative analyses were performed with the use of LC-MS/MS. The beatine was studied by a hydrophilic interaction liquid chromatography. Consequently the sensitivity of developed method was high. The mass spectrometry parameters were optimized by means of a Central Composite Design. Recoveries of studed compound from beetroot were about 97% with the use of coupled ASE and SPE.. The total contents of betaine in extracts of various part of plants (juice, peel, root) have been determined and compared.
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