Aim: This study aimed to investigate the effectiveness of dental pulp stem cells (DPSCs) associated to equin collagen (gingistat) in intrabony defect regeneration compared to collagen alone. Methods: The analysis was carried out on 18 patients with periodontitis and at least one deep intrabony defect among those referred to the Section of Periodontology, C.I.R. Dental School, University of Torino (Italy). Furthermore the indication of a vital and untreated tooth extraction was needed. Radiographic intrabony defect must be ≥ 3mm and PPD (probing pocket defect) ≥ 6mm. Subjects were randomly assigned by a computer generated list to the test group (DPSCs and gingistat) and to the control group (gingistat). Random assignment resulted in 9 patients in the test group and 9 in the control one. Clinical measurements were recorded at baseline, 3 and 6 months by one masked and calibrated examiner. The Minimal Invasive Surgical Thecnique (MIST) was used. During surgery the following mesurements were collected: distance between the cemento-enamel junction to the bottom of the defect (CEJ-BD); intraosseous defect and the number of residual walls. The treatment of the root surface was performed with 24% EDTA (Ethylenediaminetetraacetic acid) for 2 minutes before the graft positioning (gingistat with stem cells or gingistat only). Eventually, the sutures were done with Gore-tex 7-0. At 6 months the following measures were taken: full mounth plaque score (FMPS), full mouth bleeding score (FMBS), PPD(probing pocket depth), radiographic intrabony defect (INTRA-rx). Results: The two groups were comparable in terms of demographic characteristics and morphology of the defects. In the Control group CAL (clinical attachment level) gain at 6 month was 2.24 ± 1.73 mm (p=0.0037), PPD reduction was 2.57 ± 1.73 mm (p=0.0017), INTRA-rx gain was 1.50 ± 1.31 mm (p=0.009), while in the Test group CAL gain was 4.00 ± 1.39 mm (p<0.0001), PPD reduction was 4.00 ± 1.63 mm (p<0.0001), INTRA-rx gain was 2.92 ± 1.80 mm (p<0.001). At 6 month the Test group, compared to the Control group, performed better in terms of PPD reduction, CAL gain, INTRA-rx with a statistically significant difference. Conclusions: in this study dental pulp stem cells with a minimal invasive surgical technique, improved clinical outcomes in terms of CAL, PPD and INTRA-rx. During the follow-up no complications as edema or infection are been noticed. Patients didn’t refer pain. Further studies are needed to confirm data.

DENTAL PULP STEM CELLS IN PERIODONTAL REGENERATION: A RANDOMIZED CONTROLLED CLINICAL TRIAL

FERRAROTTI, FRANCESCO;MANAVELLA, VALERIA;ROMANO, Federica;AIMETTI, Mario
2014

Abstract

Aim: This study aimed to investigate the effectiveness of dental pulp stem cells (DPSCs) associated to equin collagen (gingistat) in intrabony defect regeneration compared to collagen alone. Methods: The analysis was carried out on 18 patients with periodontitis and at least one deep intrabony defect among those referred to the Section of Periodontology, C.I.R. Dental School, University of Torino (Italy). Furthermore the indication of a vital and untreated tooth extraction was needed. Radiographic intrabony defect must be ≥ 3mm and PPD (probing pocket defect) ≥ 6mm. Subjects were randomly assigned by a computer generated list to the test group (DPSCs and gingistat) and to the control group (gingistat). Random assignment resulted in 9 patients in the test group and 9 in the control one. Clinical measurements were recorded at baseline, 3 and 6 months by one masked and calibrated examiner. The Minimal Invasive Surgical Thecnique (MIST) was used. During surgery the following mesurements were collected: distance between the cemento-enamel junction to the bottom of the defect (CEJ-BD); intraosseous defect and the number of residual walls. The treatment of the root surface was performed with 24% EDTA (Ethylenediaminetetraacetic acid) for 2 minutes before the graft positioning (gingistat with stem cells or gingistat only). Eventually, the sutures were done with Gore-tex 7-0. At 6 months the following measures were taken: full mounth plaque score (FMPS), full mouth bleeding score (FMBS), PPD(probing pocket depth), radiographic intrabony defect (INTRA-rx). Results: The two groups were comparable in terms of demographic characteristics and morphology of the defects. In the Control group CAL (clinical attachment level) gain at 6 month was 2.24 ± 1.73 mm (p=0.0037), PPD reduction was 2.57 ± 1.73 mm (p=0.0017), INTRA-rx gain was 1.50 ± 1.31 mm (p=0.009), while in the Test group CAL gain was 4.00 ± 1.39 mm (p<0.0001), PPD reduction was 4.00 ± 1.63 mm (p<0.0001), INTRA-rx gain was 2.92 ± 1.80 mm (p<0.001). At 6 month the Test group, compared to the Control group, performed better in terms of PPD reduction, CAL gain, INTRA-rx with a statistically significant difference. Conclusions: in this study dental pulp stem cells with a minimal invasive surgical technique, improved clinical outcomes in terms of CAL, PPD and INTRA-rx. During the follow-up no complications as edema or infection are been noticed. Patients didn’t refer pain. Further studies are needed to confirm data.
XXI Congresso Nazionale Collegio Docenti in Odontoiatria
Roma
10-12 aprile 2014
Minerva Stomatologica
4
suppl. 1
466
466
Barbero, F.; Ferrarotti, F.; Manavella, V.; Romano, F.; Gamba, M.N.; Quirico, A.; Pigella, E.; Mariani, G.M.; Aimetti, M.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1574641
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