With Illumina NGS sequencing we were able to reconstruct a good draft for S. melongena genome. The integration of BioNano Next Generation Mapping (NGM) data increased exceptionally the quality of the genetic sequence The NGS/NGM hybrid approach not only increased the length of the scaffolds, it also extensively reduced the fragmentation of the assembly, covering over 1.2 Gbp of genome sequence with only 469 superscaffolds, and increased the quality of chromosomes reconstruction, anchoring more than 1.14 Gbp, 97% of the expected genome size.

Solanum melongena: an Illumina/BioNano Genomics integrated approach for a high quality assembly of the Eggplant genome

BARCHI, Lorenzo;COMINO, CINZIA;ACQUADRO, Alberto;PORTIS, Ezio;RINALDI, RICCARDO;SCAGLIONE, DAVIDE;LANTERI, Sergio;
2016

Abstract

With Illumina NGS sequencing we were able to reconstruct a good draft for S. melongena genome. The integration of BioNano Next Generation Mapping (NGM) data increased exceptionally the quality of the genetic sequence The NGS/NGM hybrid approach not only increased the length of the scaffolds, it also extensively reduced the fragmentation of the assembly, covering over 1.2 Gbp of genome sequence with only 469 superscaffolds, and increased the quality of chromosomes reconstruction, anchoring more than 1.14 Gbp, 97% of the expected genome size.
The 4th Plant Genomics Congress: Europe
London, UK
9-10 May 2016
The 4th Plant Genomics Congress: Europe - Utilizing NGS, omic and gene editing technologies as a tool for progressing plant research
Global Engage Ltd
9
9
Delledonne, M.; Dal Molin, A.; Minio, A.; Ferrarini, A.; Venturini, L.; Avanzato, C.; Toppino, L.; Sala, T.; Bassolino, L.; Barchi, L.; Comino, C.; Acquadro, A.; Portis, E.; Rinaldi, R.; Scaglione, D.; Francese, G.; D’Alessandro, A.; Mennella, G.; Perrone, D.; Acciarri, N.; Pietrella, M.; Aprea, G.; Sulli, M.; Lanteri, S.; Rotino, G.L.; Giuliano, G.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1595497
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