With Illumina NGS sequencing we were able to reconstruct a good draft for S. melongena genome. The integration of BioNano Next Generation Mapping (NGM) data increased exceptionally the quality of the genetic sequence The NGS/NGM hybrid approach not only increased the length of the scaffolds, it also extensively reduced the fragmentation of the assembly, covering over 1.2 Gbp of genome sequence with only 469 superscaffolds, and increased the quality of chromosomes reconstruction, anchoring more than 1.14 Gbp, 97% of the expected genome size.
Solanum melongena: an Illumina/BioNano Genomics integrated approach for a high quality assembly of the Eggplant genome
BARCHI, Lorenzo;COMINO, CINZIA;ACQUADRO, Alberto;PORTIS, Ezio;RINALDI, RICCARDO;SCAGLIONE, DAVIDE;LANTERI, Sergio;
2016-01-01
Abstract
With Illumina NGS sequencing we were able to reconstruct a good draft for S. melongena genome. The integration of BioNano Next Generation Mapping (NGM) data increased exceptionally the quality of the genetic sequence The NGS/NGM hybrid approach not only increased the length of the scaffolds, it also extensively reduced the fragmentation of the assembly, covering over 1.2 Gbp of genome sequence with only 469 superscaffolds, and increased the quality of chromosomes reconstruction, anchoring more than 1.14 Gbp, 97% of the expected genome size.
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