Certification of forest reproductive plant material is ruled by International (OECD) as well as national directions aimed to encourage the production and use of seeds, part of plants and plants that have been collected, transported, processed, raised and distributed in a manner that ensures their trueness-to-name. Certification of plant material is thus essentially based on accuracy in the handling of the plant material along the production line, given that material fits the requirements as concern its origin, reproductive technique applied and that has undergone proper selection procedures. Techniques based on the analysis of molecular markers are a powerful tool for the fingerprinting of individuals and can be used reliably for the identification of the different types of forest reproductive material. Clonally propagated material can be identified with high efficacy using SSR markers, once it is known the genetic profile of the reference clone. Sexually propagated material is also being analysed with different types of markers; individuals, as well as group of individuals, can be assigned to a population of origin by softwares using statistical algorithms, given that the genetic structure of the reference population is known. The application of molecular techniques to certification, although still theoretical, is possible and desirable. The perspectives for their employment along the production line are the best for species used in specialised plantations and clonally propagated, such as poplar, but the way still requires several steps that involve an International effort to choose and set up normalised methods of analysis.

Molecular markers and certification

BOTTA, Roberto;TORELLO MARINONI, Daniela;BOUNOUS, Giancarlo
2004-01-01

Abstract

Certification of forest reproductive plant material is ruled by International (OECD) as well as national directions aimed to encourage the production and use of seeds, part of plants and plants that have been collected, transported, processed, raised and distributed in a manner that ensures their trueness-to-name. Certification of plant material is thus essentially based on accuracy in the handling of the plant material along the production line, given that material fits the requirements as concern its origin, reproductive technique applied and that has undergone proper selection procedures. Techniques based on the analysis of molecular markers are a powerful tool for the fingerprinting of individuals and can be used reliably for the identification of the different types of forest reproductive material. Clonally propagated material can be identified with high efficacy using SSR markers, once it is known the genetic profile of the reference clone. Sexually propagated material is also being analysed with different types of markers; individuals, as well as group of individuals, can be assigned to a population of origin by softwares using statistical algorithms, given that the genetic structure of the reference population is known. The application of molecular techniques to certification, although still theoretical, is possible and desirable. The perspectives for their employment along the production line are the best for species used in specialised plantations and clonally propagated, such as poplar, but the way still requires several steps that involve an International effort to choose and set up normalised methods of analysis.
2004
Workshop of Forest Biotechnology
Conception, Cile,
2-5 marzo 2004.
Global Biotechnology Forum, Workshop of Forest Biotechnology. Conception, Cile, 2-5 marzo
University of Conception
63
72
forestry; reproductive material; DNA; SSR; cultivar; clone; GMO; identification; fingerprinting
BOTTA R; TORELLO MARINONI D.; BOUNOUS G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/17276
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