Lysozyme immobilization on cellulose-based materials as a tool for food protection

In our study, we addressed binding and controlled release of lysozyme from paper prepared from different components, and in the presence/absence of carboxymethyl cellulose (CMC). The stability of lysozyme binding to various agents (chaotropes, detergents, and salts) was also assessed. Incorporation of CMC in papers signifi-cantly increased the amount of bound lysozyme and the stability of the binding to repeated washing with buffer or with buffered chaotropes, but decreased the stability of binding towards anionic detergents. As expected, high salt concentra-tions resulted in complete removal of the protein.