In this study, the complex volatilome of maize silage samples conserved for 229 d, inoculated with Lentilactobacillus buchneri (Lbuc) and Lacticaseibacillus paracasei (Lpar), is explored by means of advanced fingerprinting methodologies based on comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry. The combined untargeted and targeted (UT) fingerprinting strategy covers 452 features, 269 of which were putatively identified and assigned within their characteristic classes. The high amounts of short-chain free fatty acids and alcohols were produced by fermentation and led to a large number of esters. The impact of Lbuc fermentation was not clearly distinguishable from the control samples; however, Lpar had a strong and distinctive signature that was dominated by propionic acid and 1-propanol characteristic volatiles. The approach provides a better understanding of silage stabilization mechanisms against the degradative action of yeasts and molds during the exposure of silage to air.

Effect of inoculation with Lentilactobacillus buchneri and Lacticaseibacillus paracasei on the maize silage volatilome: the advantages of advanced 2d-chromatographic fingerprinting approaches

Squara S.;Ferrero F.
Co-first
;
Tabacco E.;Cordero C.
;
Borreani G.
Last
2022-01-01

Abstract

In this study, the complex volatilome of maize silage samples conserved for 229 d, inoculated with Lentilactobacillus buchneri (Lbuc) and Lacticaseibacillus paracasei (Lpar), is explored by means of advanced fingerprinting methodologies based on comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry. The combined untargeted and targeted (UT) fingerprinting strategy covers 452 features, 269 of which were putatively identified and assigned within their characteristic classes. The high amounts of short-chain free fatty acids and alcohols were produced by fermentation and led to a large number of esters. The impact of Lbuc fermentation was not clearly distinguishable from the control samples; however, Lpar had a strong and distinctive signature that was dominated by propionic acid and 1-propanol characteristic volatiles. The approach provides a better understanding of silage stabilization mechanisms against the degradative action of yeasts and molds during the exposure of silage to air.
2022
70
38
12232
12248
comprehensive two-dimensional gas chromatography; combined untargeted and targeted (UT) fingerprinting; maize silage volatile organic compounds; fermentative profile LAB inocula; aerobic stability; yeast activity inhibition
Squara S., Ferrero F., Tabacco E., Cordero C., Borreani G.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1875818
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