Introduction: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by pro- gressive decline in cognitive functions leading to memory loss and dementia. Only a minority of AD cases have a genetic inheritance and are referred to as familial AD, most (90%) of them are sporadic. Among the environmental risk factors that may promote the development of AD, persis- tent brain infections, particularly those induced by herpes simplex virus-1 (HSV-1), seem to play a key role in AD pathogenesis although the underlying mechanisms have not been fully elucidated yet. Tightly connected with AD etiopathogenesis is an alteration of lipid metabolism. Recently, CMS121 was shown to protect transgenic AD mice by reducing cognitive loss and inflammation. Interestingly, fatty acid synthase (FAS), a key enzyme in the synthesis of lipids that is increased in AD patients, was identified as a target of CMS121. Considering that cellular lipid metabolism plays a pivotal role in viral infections and that the mechanisms for the metabolic reprogramming by HSV-1 are still poorly understood, we aim at dissecting the host metabolic pathways modulat- ed by HSV-1 in a neuronal-like cell line to identify new targets to prevent AD. Methods: The experiments were performed in SH-SY5Y neuronal-like cells. The cells were success- fully infected with HSV-1, thus representing a suitable in vitro model of HSV-1-associated neuronal pathologies. Next, cells were treated with different compounds (i.e., CMS121, C75, SSO) targeting lipid metabolism to test the antiviral activity. Moreover, to understand the link between FAS and HSV-1 infectivity and rule out any off-target effects of the inhibitors, FAS gene expression was silenced by specific short hairpin RNA (shRNA). Results and Conclusion: We demonstrated the capability of HSV-1 to upregulate the expression of the main lipogenesis enzymes. Accordingly, lipidomic analyses revealed an increase in both de novo synthesis and lipid storage following HSV-1 infection, confirming the role of HSV-1 in the deregulation of lipogenesis. In addition, we showed that the virus was still able to replicate upon treatment with compounds, but with reduced infectivity. Thus, we may speculate an antiviral activity of the drugs during virion assembly, leading to a block of virus production. Overall, our data unveil new aspects of HSV-1-AD interplay and uncover new potential targets to rethink new possible therapies for AD.

Exploiting lipid metabolism by HSV-1: a challenge to rethink new therapies for Alzheimer’s disease

L. Trifirò;C. Albano;S. Pasquero;F. Gugliesi;E. Mina;P. Porporato;S. Landolfo;M. De Andrea;M. Biolatti
2023-01-01

Abstract

Introduction: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by pro- gressive decline in cognitive functions leading to memory loss and dementia. Only a minority of AD cases have a genetic inheritance and are referred to as familial AD, most (90%) of them are sporadic. Among the environmental risk factors that may promote the development of AD, persis- tent brain infections, particularly those induced by herpes simplex virus-1 (HSV-1), seem to play a key role in AD pathogenesis although the underlying mechanisms have not been fully elucidated yet. Tightly connected with AD etiopathogenesis is an alteration of lipid metabolism. Recently, CMS121 was shown to protect transgenic AD mice by reducing cognitive loss and inflammation. Interestingly, fatty acid synthase (FAS), a key enzyme in the synthesis of lipids that is increased in AD patients, was identified as a target of CMS121. Considering that cellular lipid metabolism plays a pivotal role in viral infections and that the mechanisms for the metabolic reprogramming by HSV-1 are still poorly understood, we aim at dissecting the host metabolic pathways modulat- ed by HSV-1 in a neuronal-like cell line to identify new targets to prevent AD. Methods: The experiments were performed in SH-SY5Y neuronal-like cells. The cells were success- fully infected with HSV-1, thus representing a suitable in vitro model of HSV-1-associated neuronal pathologies. Next, cells were treated with different compounds (i.e., CMS121, C75, SSO) targeting lipid metabolism to test the antiviral activity. Moreover, to understand the link between FAS and HSV-1 infectivity and rule out any off-target effects of the inhibitors, FAS gene expression was silenced by specific short hairpin RNA (shRNA). Results and Conclusion: We demonstrated the capability of HSV-1 to upregulate the expression of the main lipogenesis enzymes. Accordingly, lipidomic analyses revealed an increase in both de novo synthesis and lipid storage following HSV-1 infection, confirming the role of HSV-1 in the deregulation of lipogenesis. In addition, we showed that the virus was still able to replicate upon treatment with compounds, but with reduced infectivity. Thus, we may speculate an antiviral activity of the drugs during virion assembly, leading to a block of virus production. Overall, our data unveil new aspects of HSV-1-AD interplay and uncover new potential targets to rethink new possible therapies for AD.
2023
7th National Congress of the Italian Society for Virology
Brescia, Italia
25-27 Giugno 2023
7th National Congress of the Italian Society for Virology - Abstract Book
150
150
L. Trifirò, C. Albano, S. Pasquero, F. Gugliesi, G. Bajetto, W. Hewelt-Belka, E. Mina, P. Porporato, D. Cairns, D. Kaplan, A. Mullis, S. Landolfo, M....espandi
File in questo prodotto:
File Dimensione Formato  
ABSTRACT_BOOK_SIV.pdf

Accesso aperto

Dimensione 219.29 kB
Formato Adobe PDF
219.29 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1923633
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact