The growing global demand for enhanced agricultural productivity necessitates the use of innovative breeding strategies to harness the rich genetic resources within the cultivated crops, fostering sustainability and progress. A promising avenue to reach this goal is the use of Multi-parent Advanced Generation Inter-Cross (MAGIC) population, comprising recombinant inbred lines (RILs) derived from multiple diverse founder parents, culminating in a complex genetic mosaic marked by exceptional diversity. In this study, we undertook the establishment of a MAGIC population for pepper (Capsicum annuum L.), featuring eight founder accessions distinguished by their unique phenotypic attributes. Preliminarly, we conducted a whole-genome resequencing (25x) of these parental lines. A total of about 29.6 millions polymorphisms were identified, with the 95% represented by SNPs and the 5% by InDels, and 1.2M Structural Variant (SV) were spotted. Concurrently, the eight founders were phenotyped using 23 agronomic descriptors, including resistance to the main viral pathogens: Tomato Spotted Wilt Virus (TSWV), Potato Virus Y (PVY) P0, Tobacco Mosaic Virus (TMV), and Pepper Mild Mottle Virus (PMMV) P1.2, and P1.2.3. Resistance to TSWV, TMV, and PVY was spotted across the founders, which was further screened in the MAGIC with the aim of depicting segregation patterns. The broad morpho-agronomic and the genomic characterization of the MAGIC parents are key elements to address the genetic dissection of our population. Furthermore, the resources derived from our study serves to empower the broader pepper research community, bolstering genetic and genomic investigations, and lending vital support to targeted breeding initiatives aimed at elevating the quality and productivity of pepper cultivars.
MAGIC in Capsicum annuum: Bridging phenotypic and genomic dimensions.
Martina M.;Barchi L.;Acquadro A.
;Portis E.
2024-01-01
Abstract
The growing global demand for enhanced agricultural productivity necessitates the use of innovative breeding strategies to harness the rich genetic resources within the cultivated crops, fostering sustainability and progress. A promising avenue to reach this goal is the use of Multi-parent Advanced Generation Inter-Cross (MAGIC) population, comprising recombinant inbred lines (RILs) derived from multiple diverse founder parents, culminating in a complex genetic mosaic marked by exceptional diversity. In this study, we undertook the establishment of a MAGIC population for pepper (Capsicum annuum L.), featuring eight founder accessions distinguished by their unique phenotypic attributes. Preliminarly, we conducted a whole-genome resequencing (25x) of these parental lines. A total of about 29.6 millions polymorphisms were identified, with the 95% represented by SNPs and the 5% by InDels, and 1.2M Structural Variant (SV) were spotted. Concurrently, the eight founders were phenotyped using 23 agronomic descriptors, including resistance to the main viral pathogens: Tomato Spotted Wilt Virus (TSWV), Potato Virus Y (PVY) P0, Tobacco Mosaic Virus (TMV), and Pepper Mild Mottle Virus (PMMV) P1.2, and P1.2.3. Resistance to TSWV, TMV, and PVY was spotted across the founders, which was further screened in the MAGIC with the aim of depicting segregation patterns. The broad morpho-agronomic and the genomic characterization of the MAGIC parents are key elements to address the genetic dissection of our population. Furthermore, the resources derived from our study serves to empower the broader pepper research community, bolstering genetic and genomic investigations, and lending vital support to targeted breeding initiatives aimed at elevating the quality and productivity of pepper cultivars.
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