CHROMATOGRAPHIC FUSION: AN INTEGRATED WORKFLOW FOR GC×GC-FID/MS DATA

Comprehensive two-dimensional gas chromatography (GC×GC) with parallel detection based on flame ionization (FID) and mass spectrometry (MS) is the method of choice for accurate and detailed characterization of (semi-)volatiles compounds even in the most complex matrices. FID signal ensures robust quantification with extended linearity, whereas MS trace allows reliable identification and provides the selectivity useful for solving possible co-elutions. Conventionally, the two channels must be processed independently to obtain the qualitative and quantitative analytical results of interest. This is time-consuming and demanding for the operator, yet necessary. An innovative workflow, developed on the GC Image software platform, allows merging GC×GC FID and MS plots are into a single chromatogram containing both information [1]. This fusion provides simultaneous access to quantitative and qualitative information, unlocking the ability to explore all information dimensions simultaneously. Overall analytical reliability is improved, making the workflow more accessible, streamlined, and effective. The fused chromatogram preserves the wide linear dynamic range and reproducibility of the FID while incorporating the rich information of MS, with clear benefits for a more complete, automation-ready data processing methodology. The effectiveness of the workflow has been demonstrated with both thermal and differential flow modulation, post-column splitting, or parallel secondary columns, supporting good versatility. In this study we showcase the use of this data processing approach across different fields of application.