At least 18 human genetic diseases are caused by expansion of short tandem repeats. Here we describe a successful application of a fluorescent PCR method for the detection of expanded repeats in FRDA1, SCA10, and SCA12 genes. Although this test cannot give a precise estimate of the size of the expansion, it is robust, reliable, and inexpensive, and can be used to screen large series of patients. It proved useful for confirming the presence of large expansions in the Friedreich ataxia gene following an ambiguous result of long-range PCR, as well as rapid pre-screening for large repeat expansions associated with Friedreich ataxia and SCA10 and the shorter repeat expansions associated with SCA12.

Detection of large pathogenic expansions in FRDA1, SCA10, and SCA12 genes using a simple fluorescent repeat-primed PCR assay

CAGNOLI, CLAUDIA;MIGONE, Nicola;BRUSCO, Alfredo
2004

Abstract

At least 18 human genetic diseases are caused by expansion of short tandem repeats. Here we describe a successful application of a fluorescent PCR method for the detection of expanded repeats in FRDA1, SCA10, and SCA12 genes. Although this test cannot give a precise estimate of the size of the expansion, it is robust, reliable, and inexpensive, and can be used to screen large series of patients. It proved useful for confirming the presence of large expansions in the Friedreich ataxia gene following an ambiguous result of long-range PCR, as well as rapid pre-screening for large repeat expansions associated with Friedreich ataxia and SCA10 and the shorter repeat expansions associated with SCA12.
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http://www.sciencedirect.com/science/article/pii/S1525157810604965?via%3Dihub
Friedreich ataxia; SCA10; SCA12; STR-PCR; triplet expansion
Cagnoli, Claudia; Michielotto, Chiara; Matsuura, T; Ashizawa, T; Margolis, Rl; Holmes, Se; Gellera, C; Migone, Nicola; Brusco, Alfredo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/37350
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