In our work we performed a combinatorial synthesis in aqueous medium to prepare peptide libraries from which we would select amino acid sequences with binding properties towards estrogens. We prepared an affinity solid-phase by using a tetrapeptide with good selectivity and affinity towards the estradiol (K>104 M-1). Samples of estrogens in buffer, in tap water and in river water were applied to our column in which they were retained (k>116). These could only be eluted in a few millilitres of methanol mobile phase. In all cases there were quantitative recoveries. The pre- concentration studies were promising

Chromatographic characterization of an estrogen-binding affinity column containing tetrapeptides selected by a combinatorial-binding approach

TOZZI, Cinzia;ANFOSSI, Laura;GIRAUDI, Gianfranco;GIOVANNOLI, Cristina;BAGGIANI, Claudio;VANNI, Adriano
2002

Abstract

In our work we performed a combinatorial synthesis in aqueous medium to prepare peptide libraries from which we would select amino acid sequences with binding properties towards estrogens. We prepared an affinity solid-phase by using a tetrapeptide with good selectivity and affinity towards the estradiol (K>104 M-1). Samples of estrogens in buffer, in tap water and in river water were applied to our column in which they were retained (k>116). These could only be eluted in a few millilitres of methanol mobile phase. In all cases there were quantitative recoveries. The pre- concentration studies were promising
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C. Tozzi; L. Anfossi; G. Giraudi; C. Giovannoli; C. Baggiani; A. Vanni
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/39349
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