Acinetobacter radioresistens S13, a bacterial strain selected for its ability to biodegrade phenol (qphenol = 8.87 mmol/g/h), biosynthesizes, during growth on aromatic carbon sources, a number of proteins which are absent in control conditions (sodium acetate coltures). The aim of the present research was to identify proteins, specifically induced by aromatic substrates (benzoate and/or phenol), having alkaline isoelectric points (between 6 and 11), thus integrating results obtained in previous works (Giuffrida et al., 2001; Pessione et al., 2003), concerning the analysis of auxiliary, citosolic and membrane, proteins with acidic pI (pH 4-7). The comparative proteome analysis showed 25 spots differentially expressed: at present 6 of them have been identified by ESI-MS/MS spectrometry. The biological role of the 6 identified proteins can be led back to 4 classes: 1) Proteins involved in uptake of nutrients inside the cell or in extrusion of toxic molecules (tsp Protease); 2) Proteins involved in attenuation of direct and indirect toxic effects of aromatic molecules on bacterial cells (tsp protease, S2 protein of the 30S ribosomal subunit , pseudouridine synthase  subunit); 3) Proteins connected with energy metabolism (soluble transhydrogenase for pyridine cofactors, ATP synthase  subunit); 4) Proteins connected with pH homeostatis (glutaminase/asparaginase). These results improved our knowledge on the physiology of this Acinetobacter radioresistens strain by giving new insights on regulation and adaptation mechanisms of bacterial cells to aromatic exposure.

Alkaline proteome analysis of Acinetobacter radioresistensS13

FATTORI, Paolo;MAZZOLI, Roberto;LAMBERTI, Cristina;PESSIONE, Enrica;GIUNTA, Carlo
2006

Abstract

Acinetobacter radioresistens S13, a bacterial strain selected for its ability to biodegrade phenol (qphenol = 8.87 mmol/g/h), biosynthesizes, during growth on aromatic carbon sources, a number of proteins which are absent in control conditions (sodium acetate coltures). The aim of the present research was to identify proteins, specifically induced by aromatic substrates (benzoate and/or phenol), having alkaline isoelectric points (between 6 and 11), thus integrating results obtained in previous works (Giuffrida et al., 2001; Pessione et al., 2003), concerning the analysis of auxiliary, citosolic and membrane, proteins with acidic pI (pH 4-7). The comparative proteome analysis showed 25 spots differentially expressed: at present 6 of them have been identified by ESI-MS/MS spectrometry. The biological role of the 6 identified proteins can be led back to 4 classes: 1) Proteins involved in uptake of nutrients inside the cell or in extrusion of toxic molecules (tsp Protease); 2) Proteins involved in attenuation of direct and indirect toxic effects of aromatic molecules on bacterial cells (tsp protease, S2 protein of the 30S ribosomal subunit , pseudouridine synthase  subunit); 3) Proteins connected with energy metabolism (soluble transhydrogenase for pyridine cofactors, ATP synthase  subunit); 4) Proteins connected with pH homeostatis (glutaminase/asparaginase). These results improved our knowledge on the physiology of this Acinetobacter radioresistens strain by giving new insights on regulation and adaptation mechanisms of bacterial cells to aromatic exposure.
31st FEBS Congress
Istanbul
June 24 – 29, 2006
-
BLACKWELL PUBLISHING
273
254
254
Fattori P.; Mazzoli R.; Giuffrida M.G.; Lamberti C.; Barello C.; Pessione E.; Giunta C.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/52406
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