A 60 MEA system (MCS GmbH, Reutlingen, Germany) was used for recording spontaneous action po-tentials from rat chromaffin cells (RCCs). The results obtained were compared with the intracellular ac-tion potential recorded in perforated patch clamp experiments with the aim of: 1) monitoring the spontaneous electrical activity by means of extracellular recording and, 2) identifying the role of ion chan-nels controlling action potential firings by analyzing the changes of extracellular recordings induced by selective ion channel blockers. Preliminary analysis of spontaneously active RCCs revealed the existence of strict correspondence between extracellular MEA signals and intracellular action potential recordings, making the MEA system a valuable tool for studying the functional role of ion channels in cell excitability.

Microelectrode array (MEA) analysis of ion channels controlling spontaneous chromaffin cells electrical activity

MARCANTONI, Andrea;CARABELLI, Valentina;COMUNANZA, Valentina;GAVELLO, DANIELA;CARBONE, Emilio
2008

Abstract

A 60 MEA system (MCS GmbH, Reutlingen, Germany) was used for recording spontaneous action po-tentials from rat chromaffin cells (RCCs). The results obtained were compared with the intracellular ac-tion potential recorded in perforated patch clamp experiments with the aim of: 1) monitoring the spontaneous electrical activity by means of extracellular recording and, 2) identifying the role of ion chan-nels controlling action potential firings by analyzing the changes of extracellular recordings induced by selective ion channel blockers. Preliminary analysis of spontaneously active RCCs revealed the existence of strict correspondence between extracellular MEA signals and intracellular action potential recordings, making the MEA system a valuable tool for studying the functional role of ion channels in cell excitability.
6th Int. Meeting on Substrate-Integrated Microelectrodes
Reutlingen
8-11 Luglio 2008
Proceedings MEA Meeting 2008
BIOPRO Edition
5
70
71
3938345055
http://www.nmi1.de/images/publikationen/MEA%202008%20Proceedings%20final_web.pdf
Potenziali d'azione extracellulari; eccitabilità cellulare; canli ionici; matrice di microelettrodi
Marcantoni A; Carabelli V; Comunanza V; Gavello D; Rojo-Ruiz J; Carbone E
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/71675
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact