Adjuvant arthritis (AA) was induced in Lewis rats by a single intradermal injection of Mycobacterium butyricum in Freund′s adjuvant. The experiments included healthy animals, arthritic animals not treated, and arthritic animals treated with coenzyme Q10 (CoQ10) and methotrexate (MTX). We studied CoQ10 in a daily oral dose of 20mg/kg b.w. alone and in the combinatory therapy with MTX in the oral dose of 0.3 mg/kg b.w. twice a week. Monotherapy with MTX was performed as a reference treatment. All measurements were performed on day 28 after induction of arthritis. We assessed the clinical parameter change of hind paw volume (HPV) along with biochemical characteristics-the plasmatic levels of protein carbonyl groups (ELISA) and of HNE- and MDA-protein adducts. Functionality of neutrophils was assessed by flow cytometry. HPV changes well correlated with all oxidative stress parameters. The combined CoQ10 and MTX treatment normalized all oxidative stress and AA clinical parameters, being more effective than each individual substance by itself. Moreover, the combination of MTX with CoQ10 significantly reduced MTX-induced suppression of neutrophil′s phagocytosis, correspondingly improving neutrophils'metabolic activity. These findings indicate the importance of oxidative stress in the pathogenesis of arthritis as well as the beneficial use of antioxidants in its therapy.
Methotrexate-based combinatory therapy of adjuvant arthritis. Effect of coenzyme Q10 supplementation
BIASI, Fiorella;POLI, Giuseppe
2009-01-01
Abstract
Adjuvant arthritis (AA) was induced in Lewis rats by a single intradermal injection of Mycobacterium butyricum in Freund′s adjuvant. The experiments included healthy animals, arthritic animals not treated, and arthritic animals treated with coenzyme Q10 (CoQ10) and methotrexate (MTX). We studied CoQ10 in a daily oral dose of 20mg/kg b.w. alone and in the combinatory therapy with MTX in the oral dose of 0.3 mg/kg b.w. twice a week. Monotherapy with MTX was performed as a reference treatment. All measurements were performed on day 28 after induction of arthritis. We assessed the clinical parameter change of hind paw volume (HPV) along with biochemical characteristics-the plasmatic levels of protein carbonyl groups (ELISA) and of HNE- and MDA-protein adducts. Functionality of neutrophils was assessed by flow cytometry. HPV changes well correlated with all oxidative stress parameters. The combined CoQ10 and MTX treatment normalized all oxidative stress and AA clinical parameters, being more effective than each individual substance by itself. Moreover, the combination of MTX with CoQ10 significantly reduced MTX-induced suppression of neutrophil′s phagocytosis, correspondingly improving neutrophils'metabolic activity. These findings indicate the importance of oxidative stress in the pathogenesis of arthritis as well as the beneficial use of antioxidants in its therapy.
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