INTRODUCTION AND AIMS: Polyomavirus-associated nephropathy (PVAN) is one of the most common viral diseases affecting renal allograft, with BK being the most frequent causal agent. JCV is considered responsible in <3% of the cases, while the role of SV40 is still unknown. Objectives. To quantify polyomaviruses (BK, JC and SV40) load by real-time TaqMan PCR in tissue specimens (renal and ureteral) from kidney transplant recipients. METHODS: One-hundred-thirty-eight specimens (125 kidneys, 13 ureters) obtained from 109 patients were evaluated by quantitative real-time PCR for the detection of BK, JC, and SV40-DNA. Demographic, virological, and histopathological data were collected. RESULTS: BKV-DNA was positive in 32 of 109 patients (29.6%); JCV-DNA in 20 of 109 patients (18.3%); and SV40-DNA in 11 of 109 patients (10.1%). BK viral load was >4 x 106genome equivalents/g of extracted DNA in two renal samples with histopathologically confirmed PVAN; JC viral load was >4 x 106genome equivalents/g in one ureteral sample from a patient with ureteral stenosis, while SV40 load was low in all the cases. SV-40 was detected only in concomitance with BK and/or JC. CONCLUSIONS: A complete and early diagnosis of polyomavirus-associated nephropathy seems to require the integration of multiple techniques. Our data confirm the renotropism of polyomaviruses BK and JC, as well as SV40. SV-40 was detected also in patients who did not receive the contaminated polio vaccine, confirming the occurrence of new infections due to unknown modes of transmission.

Real-time Pcr to detect polyomavirus BK-,JC- and SV40-Dna in renal and ureteral specimens from transplant recipients

Cristina Costa;BERGALLO, Massimiliano;SIDOTI, Francesca;MERLINO, Chiara;TERLIZZI, Maria Elena;SEGOLONI, Giuseppe;CAVALLO, Rossana
2009

Abstract

INTRODUCTION AND AIMS: Polyomavirus-associated nephropathy (PVAN) is one of the most common viral diseases affecting renal allograft, with BK being the most frequent causal agent. JCV is considered responsible in <3% of the cases, while the role of SV40 is still unknown. Objectives. To quantify polyomaviruses (BK, JC and SV40) load by real-time TaqMan PCR in tissue specimens (renal and ureteral) from kidney transplant recipients. METHODS: One-hundred-thirty-eight specimens (125 kidneys, 13 ureters) obtained from 109 patients were evaluated by quantitative real-time PCR for the detection of BK, JC, and SV40-DNA. Demographic, virological, and histopathological data were collected. RESULTS: BKV-DNA was positive in 32 of 109 patients (29.6%); JCV-DNA in 20 of 109 patients (18.3%); and SV40-DNA in 11 of 109 patients (10.1%). BK viral load was >4 x 106genome equivalents/g of extracted DNA in two renal samples with histopathologically confirmed PVAN; JC viral load was >4 x 106genome equivalents/g in one ureteral sample from a patient with ureteral stenosis, while SV40 load was low in all the cases. SV-40 was detected only in concomitance with BK and/or JC. CONCLUSIONS: A complete and early diagnosis of polyomavirus-associated nephropathy seems to require the integration of multiple techniques. Our data confirm the renotropism of polyomaviruses BK and JC, as well as SV40. SV-40 was detected also in patients who did not receive the contaminated polio vaccine, confirming the occurrence of new infections due to unknown modes of transmission.
World Congress of Nephrology
Milano
22/05/2009
2
1453
1453
Franca Giacchino; Cristina Costa; Massimiliano Bergallo; Francesca Sidoti; Chiara Merlino; Franco Bonello; Sara Astegiano; Maria Elena Terlizzi; Giuseppe Paolo Segoloni; Rossana Cavallo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/76416
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