The bacterium Bacillus thuringiensis subsp. israelensis produces a crystalline parasporal inclusion, called delta-endotoxin, that is used as a microbial insecticide. In our laboratory we fed fourth instar larvae of Culex pipiens with a delta-endotoxin solution lethal in 3 hours. After 10, 20 and 90 minutes, histochemical and biochemical assays were done to asses brush border LeucilAminoPeptidase involvement in the toxical action.
Leucil-aminopeptidase is involved in the toxicity to the Bacillus thuringensis subsp. israelensis delta-endotoxin in Culex pipiens larvae (Insecta, Diptera).
DORE, Bruno Emilio;ANDREOLETTI, Gian Emilio;BONELLI, Simona;MELLANO, Maria Gabriella;
1999-01-01
Abstract
The bacterium Bacillus thuringiensis subsp. israelensis produces a crystalline parasporal inclusion, called delta-endotoxin, that is used as a microbial insecticide. In our laboratory we fed fourth instar larvae of Culex pipiens with a delta-endotoxin solution lethal in 3 hours. After 10, 20 and 90 minutes, histochemical and biochemical assays were done to asses brush border LeucilAminoPeptidase involvement in the toxical action.
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