Mycobacteriul1l bo"is, the etiological agent of bovine tuberculosis (TB), has an exeeptionally IVide host range and a complex epidemiological pattem of infection by affecting domestic, farmed, •frec-ranging, and IVildlife animals, as IVell as humans. M. bo"i" is naturally resistant to pyrazinamide and usually susceptible to most antibiotics used to treat human tuberculosis. in Italy TB is stili a major concern in southem and insular regions, despite the eradication plans have been enforced for more than IO years. A preliminary study. far evaluating the susceptibility of M. bo"is isolates from animals in SiciIy, IVas carried out tOlVards the first line antibiotics used to treat human tuberculosis. On that account. 25 M, bo"i" isolates from sheep and autoehthonous pigs IVith granulomatous lesions compatible IVith TB, IVere test ed far their antimicrobial resistance to ethambutol (EB). isoniazid (IZ), rifampin (RI), streptomycin (SM), and kanamycin (KM), by follolVing the National Committee for Clinical Laboratory Standards technical guide. The genetic characterization of resistant strains IVerc al so performed by nucleotide sequencing of target genes. Our results indicated an high prevalence of resistant strains to EB (EBR) IVhich IVas conelated to missense mutations of EmbB. an arabinosyltransferase involved in celi IVall biosynthesis. Although limited studies describe the drug-resistance (OR) in M. ho!'i", our preliminary data highlight the importance of the drug susceptibility evaluation of M. bO\-is isolates in order to understand the magnitude of in!luence of the transmission of OR or multi-OR M. bO\-is strains and to ensure the implementation of the most appropriate therapy to patients.
Assesment and genetic characterization of drug resi stance in Mycobacterium bovis strains from animals in Sicily.
CAPUCCHIO, Maria Teresa;
2010-01-01
Abstract
Mycobacteriul1l bo"is, the etiological agent of bovine tuberculosis (TB), has an exeeptionally IVide host range and a complex epidemiological pattem of infection by affecting domestic, farmed, •frec-ranging, and IVildlife animals, as IVell as humans. M. bo"i" is naturally resistant to pyrazinamide and usually susceptible to most antibiotics used to treat human tuberculosis. in Italy TB is stili a major concern in southem and insular regions, despite the eradication plans have been enforced for more than IO years. A preliminary study. far evaluating the susceptibility of M. bo"is isolates from animals in SiciIy, IVas carried out tOlVards the first line antibiotics used to treat human tuberculosis. On that account. 25 M, bo"i" isolates from sheep and autoehthonous pigs IVith granulomatous lesions compatible IVith TB, IVere test ed far their antimicrobial resistance to ethambutol (EB). isoniazid (IZ), rifampin (RI), streptomycin (SM), and kanamycin (KM), by follolVing the National Committee for Clinical Laboratory Standards technical guide. The genetic characterization of resistant strains IVerc al so performed by nucleotide sequencing of target genes. Our results indicated an high prevalence of resistant strains to EB (EBR) IVhich IVas conelated to missense mutations of EmbB. an arabinosyltransferase involved in celi IVall biosynthesis. Although limited studies describe the drug-resistance (OR) in M. ho!'i", our preliminary data highlight the importance of the drug susceptibility evaluation of M. bO\-is isolates in order to understand the magnitude of in!luence of the transmission of OR or multi-OR M. bO\-is strains and to ensure the implementation of the most appropriate therapy to patients.
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