Grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. In the present study, bacterial species associated with sound and Botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. Isolates were identified by PCR–Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and sequence analysis of partial 16 S rRNA gene. Comparable counts were recorded between Botrytis-infected and sound grape samples. In all cases, the majority of isolates belonged to different species of Enterobacteriaceae. The dominant species in the vineyard was Klebsiella oxytoca that was found in different combinations with Citrobacter freundii, Enterobacter spp., Erwinia sp., Pantoea dispersa, Tatumella ptyseos or other species. In fermenting musts, those populations declined while other species evolved, like Lactobacillus plantarum and Enterobacter ludwigii. Populations in botrytised samples persisted longer during spontaneous fermentations. Present study suggests that bacterial diversity on grapes may be wider than previously described.

Bacterial species associated with sound and Botrytis-infected grapes from a Greek vineyard

RANTSIOU, KALLIOPI;COCOLIN, Luca Simone;
2011

Abstract

Grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. In the present study, bacterial species associated with sound and Botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. Isolates were identified by PCR–Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and sequence analysis of partial 16 S rRNA gene. Comparable counts were recorded between Botrytis-infected and sound grape samples. In all cases, the majority of isolates belonged to different species of Enterobacteriaceae. The dominant species in the vineyard was Klebsiella oxytoca that was found in different combinations with Citrobacter freundii, Enterobacter spp., Erwinia sp., Pantoea dispersa, Tatumella ptyseos or other species. In fermenting musts, those populations declined while other species evolved, like Lactobacillus plantarum and Enterobacter ludwigii. Populations in botrytised samples persisted longer during spontaneous fermentations. Present study suggests that bacterial diversity on grapes may be wider than previously described.
145
432
436
Grape must fermentation; bacteria; Botrytis; grapes; wine
A.A. NISIOTOU; K. RANTSIOU; V. ILIOPOULOS; L. COCOLIN; G.-J.E. NYCHAS
File in questo prodotto:
File Dimensione Formato  
IJFM, 2011, 145, 432-436.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 266.34 kB
Formato Adobe PDF
266.34 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
IJFM, 2011, 145, 432-436_open access.pdf

Accesso aperto

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 565.7 kB
Formato Adobe PDF
565.7 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/87738
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 60
  • ???jsp.display-item.citation.isi??? 53
social impact