The study evaluate a "one-stage" procedure combining minced autologous fragments, as a viable cell source, with a resorbable scaffold composed of hyaluronic acid, fibrin glue platelet rich plasma. In vitro, chondrocytes from cartilage fragments migrate and proliferated into the scaffold better in animal than in human explanted cultures. In vivo, group "one-stage" showed better quality of repair tissue than control groups and some typical features of articular cartilage, as presence of collagen type II in extracellular matrix. Nanoindentation of goat "one-stage" samples showed mechanical properties similar with normal trochlear goat cartilage. Minced cartilage fragments in a HA/fibrin/PRP scaffold provide a viable cell source for one-stage cartilage repair in rabbit and goat model.

One stage osteochondral repair with cartilage fragments in a hyaluronic acid/fibrin glue/platelet rich plasma scaffold: in vitro human and in vivo rabbit and goat animal model.

CASTOLDI, Filippo;ROSSI, Roberto;COTTINO, Umberto;PEIRONE, Bruno;MAUTHE DEGERFELD, Mitzy;ROSSI, Paolo
2010

Abstract

The study evaluate a "one-stage" procedure combining minced autologous fragments, as a viable cell source, with a resorbable scaffold composed of hyaluronic acid, fibrin glue platelet rich plasma. In vitro, chondrocytes from cartilage fragments migrate and proliferated into the scaffold better in animal than in human explanted cultures. In vivo, group "one-stage" showed better quality of repair tissue than control groups and some typical features of articular cartilage, as presence of collagen type II in extracellular matrix. Nanoindentation of goat "one-stage" samples showed mechanical properties similar with normal trochlear goat cartilage. Minced cartilage fragments in a HA/fibrin/PRP scaffold provide a viable cell source for one-stage cartilage repair in rabbit and goat model.
9th ICRS Congress
Barcellona (Spagna)
26-29 September 2010
ICRS 2010 Abstact Book
ICRS
IX
172
-
http://www.cartilage.org/files/File/Final%20Programme%2018_07.pdf
One-stage osteochondral repair; Goat animal model; Rabbit animal model; Orthopedics
Marmotti A.;Castoldi F.; Rossi R.; Realmuto C.; Bruzzone M.; Bonasia D.E.; Cottino U.; Peirone B.; Mauthe von Degerfeld M.; Rossi P.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/99659
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