Effect of coconut water and growth regulator supplement on the in vitro propagation of Corylus avellana L.

The aim of this study was to contribute to improve proliferation and growth of hazelnut using different supplements to the basal medium. Single axillary buds from mature trees of Corylus avellana L. were cultured on the modified DKW (Driver & Kuniyuki Walnut) basal medium enriched with gibberellins, cytokinins, auxins and coconut water in several combinations for the growth of hazelnut shoot explants, cultivar ‘Tonda Gentile delle Lange’. The addition of 20% coconut water to the modified DKW medium increased the number of adventitious shoots per explant. None of the used doses of cytokinin provided similar results. The results also showed that gibberellins improved the elongation and the coconut water improved the quality of plant growth in term of plant vigour. The supplement of 3 μM gibberellic acid (GA3), 2.2 μM N6-benzyladenine (BAP), 0.02 μM Indole-3-acetic acid (IAA), was effective for increasing the growth of the shoot explants. Shoot multiplication differed with the position on the medium (horizontal or vertical).