In order to improve characters of interest during the years 2008, 2009 and 2010, an F1 offspring was created by crossing between the euro-japanese hybrid ‘Bouche de Bétizac’ (C. crenata x C. sativa) and the C. sativa cultivars ‘Madonna’ in order to construct a genetic map and isolate genes coding for traits of agronomical and technological interest, such as fruit quality, early ripening, male sterility, and resistance to biotic stresses (in particular to gall wasp, Dryocosmus kuriphilus Yasumatsu). This offspring, planted in fall 2011, consists of 250 seedlings. The molecular analyses were conducted on a sample of 96 individuals of the offspring using 80 microsatellite markers (SSR), 50 of them developed on C. crenata x C. mollissima, 15 of C. sativa and 15 of Q. suber. These analyses are being implemented by adding additional SSR and SNP markers and performing field observations on agronomic traits. NGS technologies have been used for sequencing the transcriptome of the parent cultivars. A reference library of chestnut transcriptome was realized by 454 pyro-sequencing technology (Roche). A subsequent sequencing carried out by Illumina platform is being analyzed in order to identify differentially expressed genes and detect functional SSR and SNPs.
Molecular studies to identify genes coding for traits of interest in chestnut breeding
DINI, FRANCESCA;SARTOR, CHIARA;TORELLO MARINONI, Daniela;BOTTA, Roberto
2012-01-01
Abstract
In order to improve characters of interest during the years 2008, 2009 and 2010, an F1 offspring was created by crossing between the euro-japanese hybrid ‘Bouche de Bétizac’ (C. crenata x C. sativa) and the C. sativa cultivars ‘Madonna’ in order to construct a genetic map and isolate genes coding for traits of agronomical and technological interest, such as fruit quality, early ripening, male sterility, and resistance to biotic stresses (in particular to gall wasp, Dryocosmus kuriphilus Yasumatsu). This offspring, planted in fall 2011, consists of 250 seedlings. The molecular analyses were conducted on a sample of 96 individuals of the offspring using 80 microsatellite markers (SSR), 50 of them developed on C. crenata x C. mollissima, 15 of C. sativa and 15 of Q. suber. These analyses are being implemented by adding additional SSR and SNP markers and performing field observations on agronomic traits. NGS technologies have been used for sequencing the transcriptome of the parent cultivars. A reference library of chestnut transcriptome was realized by 454 pyro-sequencing technology (Roche). A subsequent sequencing carried out by Illumina platform is being analyzed in order to identify differentially expressed genes and detect functional SSR and SNPs.
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