The hazelnut (Corylus avellana L.) hardly roots by cutting and auxins treatments are essential to induce roots formation on hazelnut cuttings. Auxins can affect the ethylene and ABA (abscisic acid) production with an effect on bud abscission and leaf drop, leading to cutting death. The aim of this study was to test the role of ethylene and ABA in these processes in cultivar ‘Tonda Gentile delle Langhe’. Herbaceous cuttings from the canopy were treated with 1000 mgL-1 of IBA (indole-3-butyric acid) and placed in air-tight glass bottles for 6 days. The ethylene levels produced by the samples were determined at 3, 6, 12, 24, 72 and 144 hours after IBA treatment using a gas chromatograph (GC). The analyses of the expression of genes involved in ACC oxidase and ABA biosynthesis were carryed out by quantitative RT-PCR analysis on cDNAs isolated from bud and leaf samples. The plant material was placed in a air-tight glass cabinet and the cuttings were sampled before IBA treatment and 1.5, 3,6, 12, 24, 72 and 144 hours after IBA treatment. Untreated cuttings were used as control.
Role of ethylene and ABA in bud abscission and leaf drop on hazelnut (Corylus avellana L.) herbaceous cuttings
CONTESSA, CECILIA;GAMBINO, Giorgio;BOCCACCI, PAOLO;SEGLIE, LUDOVICA;BOTTA, Roberto
2012-01-01
Abstract
The hazelnut (Corylus avellana L.) hardly roots by cutting and auxins treatments are essential to induce roots formation on hazelnut cuttings. Auxins can affect the ethylene and ABA (abscisic acid) production with an effect on bud abscission and leaf drop, leading to cutting death. The aim of this study was to test the role of ethylene and ABA in these processes in cultivar ‘Tonda Gentile delle Langhe’. Herbaceous cuttings from the canopy were treated with 1000 mgL-1 of IBA (indole-3-butyric acid) and placed in air-tight glass bottles for 6 days. The ethylene levels produced by the samples were determined at 3, 6, 12, 24, 72 and 144 hours after IBA treatment using a gas chromatograph (GC). The analyses of the expression of genes involved in ACC oxidase and ABA biosynthesis were carryed out by quantitative RT-PCR analysis on cDNAs isolated from bud and leaf samples. The plant material was placed in a air-tight glass cabinet and the cuttings were sampled before IBA treatment and 1.5, 3,6, 12, 24, 72 and 144 hours after IBA treatment. Untreated cuttings were used as control.
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