Oxidative stress, more precisely the damage caused by an abnormal production of reactive oxygen species (ROS), is a key mechanism in ALS pathogenesis. NADPH oxidases are a family of enzymes responsible for the production of ROS. In our previous study, an increased survival of ALS-affected individuals has been related to low activity of NADPH oxidase-2 (NOX2) in their neutrophils (1). Based on this finding, this work aimed to evaluate the protein levels of NOX2 in neutrophils of patients affected by ALS, in order to verify a possible correlation between levels and activity. The analysis was performed in a cohort of 40 subjects using Western immunoblot technique. The attention was mainly focused on ALS patients carrying mutations of copper-zinc superoxide dismutase-1 (SOD1) gene, since mutations of SOD1 are referred to alter NOX2 activity (2). (1) Marrali G et al. NADPH oxidase (NOX2) activity is a modifier of survival in ALS. J. Neurol. 2014 (2) Harraz et al. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J Clin Invest. 2008

Expression of NOX2 protein in neutrophils of patients with ALS

DE MARCO, GIOVANNI;LOMARTIRE, ANNAROSA;CASALE, Federico;MARRALI, GIUSEPPE;CAMMAROSANO, Stefania;ILARDI, ANTONIO;CANOSA, ANTONIO;MOGLIA, Cristina;CALVO, Andrea;CHIO', Adriano
2015

Abstract

Oxidative stress, more precisely the damage caused by an abnormal production of reactive oxygen species (ROS), is a key mechanism in ALS pathogenesis. NADPH oxidases are a family of enzymes responsible for the production of ROS. In our previous study, an increased survival of ALS-affected individuals has been related to low activity of NADPH oxidase-2 (NOX2) in their neutrophils (1). Based on this finding, this work aimed to evaluate the protein levels of NOX2 in neutrophils of patients affected by ALS, in order to verify a possible correlation between levels and activity. The analysis was performed in a cohort of 40 subjects using Western immunoblot technique. The attention was mainly focused on ALS patients carrying mutations of copper-zinc superoxide dismutase-1 (SOD1) gene, since mutations of SOD1 are referred to alter NOX2 activity (2). (1) Marrali G et al. NADPH oxidase (NOX2) activity is a modifier of survival in ALS. J. Neurol. 2014 (2) Harraz et al. SOD1 mutations disrupt redox-sensitive Rac regulation of NADPH oxidase in a familial ALS model. J Clin Invest. 2008
Neurinox International Symposium "Innovative Concepts for Treating Neurodegenerative Diseases"
Torino, Italia
23-25 Settembre 2015
Neurinox International Symposium - Programme and abstract booklet
Neurinox Consortium
55
55
SLA, epilessia, sclerosi multipla
De Marco G, Lomartire A, Casale F, Marrali G, Cammarosano S, Ilardi A, Canosa A, Moglia C, Calvo A, Rinaudo MT, Chiò A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1562313
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