Apoptosis is an internally programmed mechanism of cell death. Dysregulation of this process may lead to carcinogenesis, tumor progression and resistance to chemotherapy. The aim of the study was to characterize apoptosis, as determined by flow cytometry, in different canine lymphomas (LSAs) and to investigate its prognostic significance. Based on immunophenotype and updated Kiel classification, 131 canine LSAs were classified as 112 high grade (HG) LSAs (90 B-cell, 22 T-cell) and 19 low grade (LG) LSAs (7 B-cell, 12T-cell). Lymph node aspirates were stained using AnnexinVFITC/ Propidium Iodide and analysed by flow cytometry. Apoptosis was expressed as percentage of AnnexinV positive cells (% AnnV+). Statistical comparisons between groups were assessed by t-test. For Kaplan-Meier curves median values, 25th and 75th percentiles of % AnnV+ were chosen as cutoffs to evaluate overall survival (OS) and relapse free interval (RFI) in 22 B- and 10 T-cell HG LSAs. In HG LSAs % AnnV+ was significantly higher (56.8% +/- 25.9, P < .001) compared to LG LSAs (27.1% +/-23.4). B-cell LSAs (65.2% +/-18) showed significantly higher % AnnV+ than T-cell LSAs (16% +/-13.8, P < .001), also within HG and LG LSAs. Concerning prognostic evaluations, only the 75th percentile of % AnnV+ (80.3%) in HG B-cell LSAs was able to discriminate between 2 groups with a significantly different OS (P = .03). Apoptosis has been found to be higher in HG LSAs, confirming that aggressive tumors present rapid cellular turnover, and in B-cell LSAs. The distinct apoptotic activity shown by LSAs with different immunophenotype could represent a target for treatment refinement in canine lymphoma.
Flow cytometric detection of apoptotic cells in canine lymphoma
POGGI, ALESSIA;MINISCALCO, Barbara;MORELLO, Emanuela Maria;GATTINO, FRANCESCA;Aresu L;RIONDATO, Fulvio
2014-01-01
Abstract
Apoptosis is an internally programmed mechanism of cell death. Dysregulation of this process may lead to carcinogenesis, tumor progression and resistance to chemotherapy. The aim of the study was to characterize apoptosis, as determined by flow cytometry, in different canine lymphomas (LSAs) and to investigate its prognostic significance. Based on immunophenotype and updated Kiel classification, 131 canine LSAs were classified as 112 high grade (HG) LSAs (90 B-cell, 22 T-cell) and 19 low grade (LG) LSAs (7 B-cell, 12T-cell). Lymph node aspirates were stained using AnnexinVFITC/ Propidium Iodide and analysed by flow cytometry. Apoptosis was expressed as percentage of AnnexinV positive cells (% AnnV+). Statistical comparisons between groups were assessed by t-test. For Kaplan-Meier curves median values, 25th and 75th percentiles of % AnnV+ were chosen as cutoffs to evaluate overall survival (OS) and relapse free interval (RFI) in 22 B- and 10 T-cell HG LSAs. In HG LSAs % AnnV+ was significantly higher (56.8% +/- 25.9, P < .001) compared to LG LSAs (27.1% +/-23.4). B-cell LSAs (65.2% +/-18) showed significantly higher % AnnV+ than T-cell LSAs (16% +/-13.8, P < .001), also within HG and LG LSAs. Concerning prognostic evaluations, only the 75th percentile of % AnnV+ (80.3%) in HG B-cell LSAs was able to discriminate between 2 groups with a significantly different OS (P = .03). Apoptosis has been found to be higher in HG LSAs, confirming that aggressive tumors present rapid cellular turnover, and in B-cell LSAs. The distinct apoptotic activity shown by LSAs with different immunophenotype could represent a target for treatment refinement in canine lymphoma.
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