The interest in the utilization of chestnut dwarfing rootstocks is ever more growing in the high density plantations.Hybrid dwarfing rootstocks, selected for their resistance to inkdisease and canker blight too, are actually mainly propagated by layering. However, the improvement of various in vitro procedures in the last 20 years has opened thepossibility of an effective micropropagation of a number of woody species, including chestnut.The aim of this research was to develop an easy and efficient micropropagation protocol for chestnut rootstocks, suitable for a routine application incommercial nurseries.Rootstocks mother plants produced by layering at three different juvenility stages were used as starter material. The experimental protocol involved four stages:1) initiation (in vitro shoot growth of primary explants); 2) shoot proliferation; 3)shoot rooting; and 4) plantlet acclimatization (hardening). The final aim of the research, developed in collaboration with the Regional Chestnut Centre of Piemonte, is to transfer the know-how to the nurseries in order to foster a more competitive developmentof chestnut industry.
MICROPROPAGATING CHESTNUT DWARFING ROOTSTOCKS: DEFINITION OF A ROUTINE PROTOCOL
Chiavazza Paola Maria;Donno Dario;CAYRE, ELISABETTA;Mellano Maria Gabriella;Beccaro Gabriele Loris
2017-01-01
Abstract
The interest in the utilization of chestnut dwarfing rootstocks is ever more growing in the high density plantations.Hybrid dwarfing rootstocks, selected for their resistance to inkdisease and canker blight too, are actually mainly propagated by layering. However, the improvement of various in vitro procedures in the last 20 years has opened thepossibility of an effective micropropagation of a number of woody species, including chestnut.The aim of this research was to develop an easy and efficient micropropagation protocol for chestnut rootstocks, suitable for a routine application incommercial nurseries.Rootstocks mother plants produced by layering at three different juvenility stages were used as starter material. The experimental protocol involved four stages:1) initiation (in vitro shoot growth of primary explants); 2) shoot proliferation; 3)shoot rooting; and 4) plantlet acclimatization (hardening). The final aim of the research, developed in collaboration with the Regional Chestnut Centre of Piemonte, is to transfer the know-how to the nurseries in order to foster a more competitive developmentof chestnut industry.
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