Root orchestrates the defense adaptations to drought, acting as a sink of the carbon in competition with other plant organs during growth slowdowns. We aim to study C allocation kinetics in grapevine organs in a controlled drought system basing on pulse-chasing isotopic strategy. The isotope acts as a tracer of the floematic flows that are oriented towards different sinks during drought/rehydration cycles. Photosynthetic assimilation, stomatal regulation and respiration are checked in the various phases to size 13CO2 enrichment flows in the chamber. Maximum assimilation ranges from 9 to 12 μmol m-2 s-1, reduced by stomatal control 3 o 4 times at the end of the drought period. Light responses are evaluated to optimizing chamber illumination. Saturation of net photosynthesis occurs around 1000 μmol m-2 s-1 PPFD: thereafter photoinhibition impairment starts in old leaves, whereas young leaves experience half of maximum assimilation without drastic photoinhibition. Temperature effects on photorespiration are checked and considered. A model optimizing light and temperature is proposed for plants in the various stages of the experiment. Financial support: Cassa Risparmio Torino Foundation.

Design and set up of a plant growth chamber for stable isotope labeling to investigate carbon attraction toward fruit sinks and plant reserves upon and after drought stress

PATONO, DAVIDE LUCIEN;D. Said Pullicino;A. Ferrandino;L. Celi;Claudio Lovisolo
2018

Abstract

Root orchestrates the defense adaptations to drought, acting as a sink of the carbon in competition with other plant organs during growth slowdowns. We aim to study C allocation kinetics in grapevine organs in a controlled drought system basing on pulse-chasing isotopic strategy. The isotope acts as a tracer of the floematic flows that are oriented towards different sinks during drought/rehydration cycles. Photosynthetic assimilation, stomatal regulation and respiration are checked in the various phases to size 13CO2 enrichment flows in the chamber. Maximum assimilation ranges from 9 to 12 μmol m-2 s-1, reduced by stomatal control 3 o 4 times at the end of the drought period. Light responses are evaluated to optimizing chamber illumination. Saturation of net photosynthesis occurs around 1000 μmol m-2 s-1 PPFD: thereafter photoinhibition impairment starts in old leaves, whereas young leaves experience half of maximum assimilation without drastic photoinhibition. Temperature effects on photorespiration are checked and considered. A model optimizing light and temperature is proposed for plants in the various stages of the experiment. Financial support: Cassa Risparmio Torino Foundation.
Congresso nazionale SIBV FISV
Roma
18-21 September 2018
-
617
617
carbon, drought, root
D. L. Patono, D. Said Pullicino, A. Firbus, A. Ferrandino, G. Gambino, L. Celi, Claudio Lovisolo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1679986
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