Codominants, highly polymorphous and present in neighbouring species and genus, microsatellites could be considered ideal markers. In this project 42 Quercus robur and 11 Quercus petraea isolated loci have been tested for the screening of 5 cultivar DNA. Afterwards 11 loci, well amplified, with regular profiles and three alleles at least, have been tested on 12 chestnut cultivar, in order to evaluate their polymorphism. Five loci interesting for the genetic certification have been observed and used, toghether with Castanea sativa isolated loci for a multiplex analysis with a semi-automatic DNA sequencer.
Isolamento e caratterizzazione di sequenze microsatelliti in Castanea sativa Mill.
D. MARINONI;BOTTA, Roberto;BOUNOUS, Giancarlo
2002-01-01
Abstract
Codominants, highly polymorphous and present in neighbouring species and genus, microsatellites could be considered ideal markers. In this project 42 Quercus robur and 11 Quercus petraea isolated loci have been tested for the screening of 5 cultivar DNA. Afterwards 11 loci, well amplified, with regular profiles and three alleles at least, have been tested on 12 chestnut cultivar, in order to evaluate their polymorphism. Five loci interesting for the genetic certification have been observed and used, toghether with Castanea sativa isolated loci for a multiplex analysis with a semi-automatic DNA sequencer.
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