Hermetia illucens (HI) oil, extracted from black soldier fly larvae, represents a sustainable alternative to traditional fat sources in bovine feeding. However, its impact on bovine genome stability was never investigated. Sister chromatid exchange (SCE) is a biological indicator of genomic instability, useful to assess the effect of genotoxic agents. This study aimed to evaluate the effect of HI oil inclusion in the feed ration on bovine genome stability by analyzing SCE in vitro. Twenty-six Valdostana Red Pied cows fed mixed hay ad libitum were divided into two balanced groups that received isonitrogenous and isoenergetic concentrates containing a conventional lipid source, palm oil (control group), or HI oil at 3% as fed (case group). Peripheral blood lymphocytes were cultured in vitro for conventional (normal cultures) and 5′-bromodeoxyuridine (BrdU) incorporation; the latter was added 26 h before harvesting at a final concentration of 10 μg/mL to obtain preparations for the SCE test. Slides obtained from both normal cultures were karyotyped by GTG banding, whereas the BrdUtreated cultures were stained for 10 min with acridine orange (0.01% in buffer phosphate), washed with distilled water, and mounted in P-buffer. Three time points were analyzed: zero time (T0—no HI oil inclusion), 30 days (T1), and 50 days (T2) after the start of the experimental feeding. Cows were all karyologically normal (2n = 60,XX). A total of 2882 metaphases were counted for the SCE test. Statistical analysis using the Student’s t-test showed no significant differences in SCE frequency between the control and case groups at T0 (6.84 ± 0.15 vs. 6.72 ± 0.14; p = 0.53) and T1 (6.28 ± 0.12 vs. 6.27 ± 0.12; p = 0.89). However, after 50 days (T2), a significant reduction in SCE frequency was observed in the case compared to the control group (5.73 ± 0.11 vs. 6.29 ± 0.12; p = 0.002). In conclusion, results suggest that the tested dietary HI oil inclusion level does not have a negative effect on bovine genome stability in vitro. The reduction in SCE frequency in the case group appears to be a putative protective effect of the HI feed inclusion on genome stability, although further studies and other genotoxic tests are needed to confirm this trend.
Effect of Dietary Hermetia illucens Oil on Bovine Genome Stability: A Sister Chromatid Exchange (SCE) Study
Pauciullo, Alfredo
Membro del Collaboration Group
;Gaspa, Giustino;Rastello, Lara;Gasco, Laura;Renna, Manuela
2024-01-01
Abstract
Hermetia illucens (HI) oil, extracted from black soldier fly larvae, represents a sustainable alternative to traditional fat sources in bovine feeding. However, its impact on bovine genome stability was never investigated. Sister chromatid exchange (SCE) is a biological indicator of genomic instability, useful to assess the effect of genotoxic agents. This study aimed to evaluate the effect of HI oil inclusion in the feed ration on bovine genome stability by analyzing SCE in vitro. Twenty-six Valdostana Red Pied cows fed mixed hay ad libitum were divided into two balanced groups that received isonitrogenous and isoenergetic concentrates containing a conventional lipid source, palm oil (control group), or HI oil at 3% as fed (case group). Peripheral blood lymphocytes were cultured in vitro for conventional (normal cultures) and 5′-bromodeoxyuridine (BrdU) incorporation; the latter was added 26 h before harvesting at a final concentration of 10 μg/mL to obtain preparations for the SCE test. Slides obtained from both normal cultures were karyotyped by GTG banding, whereas the BrdUtreated cultures were stained for 10 min with acridine orange (0.01% in buffer phosphate), washed with distilled water, and mounted in P-buffer. Three time points were analyzed: zero time (T0—no HI oil inclusion), 30 days (T1), and 50 days (T2) after the start of the experimental feeding. Cows were all karyologically normal (2n = 60,XX). A total of 2882 metaphases were counted for the SCE test. Statistical analysis using the Student’s t-test showed no significant differences in SCE frequency between the control and case groups at T0 (6.84 ± 0.15 vs. 6.72 ± 0.14; p = 0.53) and T1 (6.28 ± 0.12 vs. 6.27 ± 0.12; p = 0.89). However, after 50 days (T2), a significant reduction in SCE frequency was observed in the case compared to the control group (5.73 ± 0.11 vs. 6.29 ± 0.12; p = 0.002). In conclusion, results suggest that the tested dietary HI oil inclusion level does not have a negative effect on bovine genome stability in vitro. The reduction in SCE frequency in the case group appears to be a putative protective effect of the HI feed inclusion on genome stability, although further studies and other genotoxic tests are needed to confirm this trend.
| File | Dimensione | Formato | |
|---|---|---|---|
|
O13 - Proceedings ICACG 2024.pdf
Accesso aperto
Descrizione: abstract
Tipo di file:
PDF EDITORIALE
Dimensione
196.75 kB
Formato
Adobe PDF
|
196.75 kB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
