A cortisol-binding polymer was prepared by utilising a non- covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-ketoprogesterone, 11alpha-hydroxyprogesterone, 17alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v:v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11alpha-hydroxyprogesterone, was found

A molecularly imprinted polymer binding cortisol

BAGGIANI, Claudio;GIRAUDI, Gianfranco;TROTTA, Francesco;GIOVANNOLI, Cristina;VANNI, Adriano
2000

Abstract

A cortisol-binding polymer was prepared by utilising a non- covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-ketoprogesterone, 11alpha-hydroxyprogesterone, 17alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v:v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11alpha-hydroxyprogesterone, was found
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C. Baggiani; G. Giraudi; F. Trotta; C. Giovannoli; A. Vanni
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/6116
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