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EnglishA cortisol-binding polymer was prepared by utilising a non- covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-ketoprogesterone, 11alpha-hydroxyprogesterone, 17alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v:v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11alpha-hydroxyprogesterone, was found
| Titolo: | A molecularly imprinted polymer binding cortisol |
| Autori Riconosciuti: | |
| Autori: | C. Baggiani; G. Giraudi; F. Trotta; C. Giovannoli; A. Vanni |
| Data di pubblicazione: | 2000 |
| Abstract: | A cortisol-binding polymer was prepared by utilising a non- covalent molecular imprinting polymerisation technique. The obtained polymer was packed in a high-performance liquid chromatography (HPLC) column; the selectivity was studied by liquid chromatography, eluting cortisol, cortisone, corticosterone, progesterone, 11-ketoprogesterone, 11alpha-hydroxyprogesterone, 17alpha-hydroxyprogesterone, cortisol 21-hemisuccinate, and cortisol 21-acetate with chloroform, containing 0.5% (v:v) acetic acid, as mobile phase. The mechanism of molecular imprinting was confirmed and a good selectivity for cortisol, with limited recognition for cortisone and 11alpha-hydroxyprogesterone, was found |
| Editore: | Elsevier Science Limited:Oxford Fulfillment Center, PO Box 800, Kidlington Oxford OX5 1DX United Kingdom:011 44 1865 843000, 011 44 1865 843699, EMAIL: asianfo@elsevier.com, tcb@elsevier.co.UK, INTERNET: http://www.elsevier.com, http://www.elsevier.com/locate/shpsa/, Fax: 011 44 1865 843010 |
| Volume: | 51 |
| Pagina iniziale: | 71 |
| Pagina finale: | 75 |
| Rivista: | TALANTA |
| Appare nelle tipologie: | 03A-Articolo su Rivista |
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http://hdl.handle.net/2318/6116
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