The European hazelnut (Corylus avellana L.) is a monoeciuos tree and exhibits sporophytic self-incompatibility. Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals. Self-incompatibility is controlled by a single multi-allelic locus, the S locus. The involved molecular mechanisms are still partly unknown but in the Brassicaceae. Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut. The Differential Display technique was applied for the study of the female determinant of self-incompatibility. Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom. The results allowed to isolate partial sequences that showed an interesting homology degree with transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the efforts for getting differentially expressed sequences of this type were increased. Primers were designed on conserved regions of serine-threonine kinase receptors. Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins. The isolated sequences are being studied to check their expression in different tissues and style developmental stages.

Gene Expression and Sporophytic Self-Incompatibility in Hazelnut

TORELLO MARINONI, Daniela;BELTRAMO, CHIARA;AKKAK, Aziz;DESTEFANIS, MARIALAURA;BOCCACCI, PAOLO;BOTTA, Roberto
2009

Abstract

The European hazelnut (Corylus avellana L.) is a monoeciuos tree and exhibits sporophytic self-incompatibility. Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals. Self-incompatibility is controlled by a single multi-allelic locus, the S locus. The involved molecular mechanisms are still partly unknown but in the Brassicaceae. Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut. The Differential Display technique was applied for the study of the female determinant of self-incompatibility. Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom. The results allowed to isolate partial sequences that showed an interesting homology degree with transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the efforts for getting differentially expressed sequences of this type were increased. Primers were designed on conserved regions of serine-threonine kinase receptors. Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins. The isolated sequences are being studied to check their expression in different tissues and style developmental stages.
VII International Congress on Hazelnut
Viterbo
23-27 giugno 2008
845
227
231
Corylus avellana L.; S locus; female determinant; Differential Display
Torello Marinoni D.; Beltramo C.; Akkak A.; Destefanis M.L.; Boccacci P.; R. Botta
File in questo prodotto:
File Dimensione Formato  
Torello Marinoni et al 2009.pdf

Accesso aperto

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 309.6 kB
Formato Adobe PDF
309.6 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/76902
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 8
  • ???jsp.display-item.citation.isi??? ND
social impact