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EnglishThe European hazelnut (Corylus avellana L.) is a monoeciuos tree and exhibits sporophytic self-incompatibility. Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals. Self-incompatibility is controlled by a single multi-allelic locus, the S locus. The involved molecular mechanisms are still partly unknown but in the Brassicaceae. Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut. The Differential Display technique was applied for the study of the female determinant of self-incompatibility. Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom. The results allowed to isolate partial sequences that showed an interesting homology degree with transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the efforts for getting differentially expressed sequences of this type were increased. Primers were designed on conserved regions of serine-threonine kinase receptors. Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins. The isolated sequences are being studied to check their expression in different tissues and style developmental stages.
| Titolo: | Gene Expression and Sporophytic Self-Incompatibility in Hazelnut |
| Autori Riconosciuti: | |
| Autori: | Torello Marinoni D.; Beltramo C.; Akkak A.; Destefanis M.L.; Boccacci P.; R. Botta |
| Data di pubblicazione: | 2009 |
| Abstract: | The European hazelnut (Corylus avellana L.) is a monoeciuos tree and exhibits sporophytic self-incompatibility. Self-incompatibility is a genetic system that prevents the self-fertilization allowing the pistil to reject the pollen of genetically close individuals. Self-incompatibility is controlled by a single multi-allelic locus, the S locus. The involved molecular mechanisms are still partly unknown but in the Brassicaceae. Studies on gene regulation of fertility, pollination and fertilization in hazelnut are very few; so with this research we propose to contribute to the knowledge about the mechanism of sporophytic self-incompatibility in hazelnut. The Differential Display technique was applied for the study of the female determinant of self-incompatibility. Two developmental stages of styles/stygmas were compared: before emergence from the bud and at full bloom. The results allowed to isolate partial sequences that showed an interesting homology degree with transmembrane serine-threonine kinase receptor of Brassica oleracea. Believing that the female determinant of self-incompatibility in hazelnut is very likely a membrane receptor, the efforts for getting differentially expressed sequences of this type were increased. Primers were designed on conserved regions of serine-threonine kinase receptors. Four differentially expressed fragments were isolated from stigmas at full bloom: after blasting in TIGR and NCBI databases, one was homologous to a gene for a kinase receptor, three were homologous to kinase proteins. The isolated sequences are being studied to check their expression in different tissues and style developmental stages. |
| Volume: | 845 |
| Pagina iniziale: | 227 |
| Pagina finale: | 231 |
| Nome del convegno: | VII International Congress on Hazelnut |
| Luogo del convegno: | Viterbo |
| Anno del convegno: | 23-27 giugno 2008 |
| Parole Chiave: | Corylus avellana L.; S locus; female determinant; Differential Display |
| Rivista: | ACTA HORTICULTURAE |
| Appare nelle tipologie: | 04B-Conference paper in rivista |
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| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| Torello Marinoni et al 2009.pdf | POSTPRINT (VERSIONE FINALE DELL’AUTORE) | Accesso aperto | Open Access Visualizza/Apri |
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