Essential oils (e.o.s) are complex mixtures of components with similar structural and physical characteristics (e.g. mono- and sesquiterpenoids...), and often difficult to identify because of their similar mass spectra. As a consequence, diagnostic chromatographic data becomes indispensable for a correct analyte identification. New GC stationary phases with different selectivity have therefore to be found. Ionic liquids (ILs) are promising as moderately-polar-topolar stationary phases for GC because their selectivity on specific chemical classes strongly differs from that of conventional phases [1-4] such as polyethylenglycols (PEG). This poster reports the results of a study about the evolution over time of the performance of commercially available IL columns in the analysis of some e.o.s of different complexity by comparing evaluated versus conventional stationary phases of similar polarity (i.e. PEG columns). The first generation of IL columns shows good selectivity depending on the analyte polarity/volatility but poor chromatographic efficiency (high adsorption, peak tailing…). This limit has been partly overcome by a second generation of IL columns with a better chromatographic efficiency, medium polarity, but lower selectivity. Further improvements are still necessary in particular to reduce adsorption of moderately polar compounds. References: [1] K. Huang et al. Anal. Bioanal. Chem. 389 (2007) 2265 [2] T. Payagala et al. Anal. Chem. 81 (2009) 160 [3] M. Qi et al. Anal. Bioanal. Chem. 388 (2007) 889 [4] C. Ragonese et al. Anal. Chem. 83 (2011) 7947
ADVANTAGES AND DISADVANTAGES OF IONIC LIQUID COATED GC COLUMNS IN ESSENTIAL OIL ANALYSIS
LIBERTO, Erica;BICCHI, Carlo;CORDERO, Chiara Emilia Irma;RUOSI, MANUELA ROSANNA;RUBIOLO, Patrizia;SGORBINI, Barbara;CAGLIERO, Cecilia Lucia
2012-01-01
Abstract
Essential oils (e.o.s) are complex mixtures of components with similar structural and physical characteristics (e.g. mono- and sesquiterpenoids...), and often difficult to identify because of their similar mass spectra. As a consequence, diagnostic chromatographic data becomes indispensable for a correct analyte identification. New GC stationary phases with different selectivity have therefore to be found. Ionic liquids (ILs) are promising as moderately-polar-topolar stationary phases for GC because their selectivity on specific chemical classes strongly differs from that of conventional phases [1-4] such as polyethylenglycols (PEG). This poster reports the results of a study about the evolution over time of the performance of commercially available IL columns in the analysis of some e.o.s of different complexity by comparing evaluated versus conventional stationary phases of similar polarity (i.e. PEG columns). The first generation of IL columns shows good selectivity depending on the analyte polarity/volatility but poor chromatographic efficiency (high adsorption, peak tailing…). This limit has been partly overcome by a second generation of IL columns with a better chromatographic efficiency, medium polarity, but lower selectivity. Further improvements are still necessary in particular to reduce adsorption of moderately polar compounds. References: [1] K. Huang et al. Anal. Bioanal. Chem. 389 (2007) 2265 [2] T. Payagala et al. Anal. Chem. 81 (2009) 160 [3] M. Qi et al. Anal. Bioanal. Chem. 388 (2007) 889 [4] C. Ragonese et al. Anal. Chem. 83 (2011) 7947
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