The present work focuses on the expression analysis of virulence and stress response genes (plcA, hly, iap and sigB) in 11 different strains of L. monocytogenes. The first step of this study considered an in vitro analysis in synthetic laboratory medium. According to statistical analysis, significant differences emerged for genes sigB and plcA. Subsequently, a soft cheese at two temperatures (4 and 12°C) and for different times (24 and 48 hours) was evaluated. Significant expression differences emerged for the condition at 12°C after 48 h. No association could be observed between gene expression profile and origin of the different strains.

A comparison of gene expression of Listeria monocytogenes in vitro and the soft cheese Crescenza

ALESSANDRIA, Valentina;RANTSIOU, KALLIOPI;DOLCI, Paola;ZEPPA, Giuseppe;COCOLIN, Luca Simone
2013-01-01

Abstract

The present work focuses on the expression analysis of virulence and stress response genes (plcA, hly, iap and sigB) in 11 different strains of L. monocytogenes. The first step of this study considered an in vitro analysis in synthetic laboratory medium. According to statistical analysis, significant differences emerged for genes sigB and plcA. Subsequently, a soft cheese at two temperatures (4 and 12°C) and for different times (24 and 48 hours) was evaluated. Significant expression differences emerged for the condition at 12°C after 48 h. No association could be observed between gene expression profile and origin of the different strains.
2013
66
83
89
Listeria monocytogenes; virulence gene expression; soft cheese; RT-qPCR
V. ALESSANDRIA; K. RANTSIOU; P. DOLCI; G. ZEPPA; L. COCOLIN
File in questo prodotto:
File Dimensione Formato  
IJDT, 2013, 66, 83-89.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 99.97 kB
Formato Adobe PDF
99.97 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
IJDT, 2013, 66, 83-89_4aperto.pdf

Accesso aperto

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 605.41 kB
Formato Adobe PDF
605.41 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/119480
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 19
  • ???jsp.display-item.citation.isi??? 15
social impact