Despite clinical findings suggesting that the form (liquid versus solid) of the sugars may significantly affect the development of metabolic diseases, no experimental data are available on the impact of their formulations on gut microbiota, integrity and hepatic outcomes. In the present sudy, C57Bl/6j mice were fed a standard diet plus water (SD), a standard diet plus 60% fructose syrup (L-Fr) or a 60% fructose solid diet plus water (S-Fr) for 12 weeks. Gut microbiota was characterized through 16S rRNA phylogenetic profiling and shotgun sequencing of microbial genes in ileum content and related volatilome profiling. Fructose feeding led to alterations of the gutmicrobiota depending on the fructose formulation,with increased colonization by Clostridium,Oscillospira and Clostridiales phyla in the S-Fr group and Bacteroides, Lactobacillus, Lachnospiraceae and Dorea in the L-Fr. S-Fr evoked the highest accumulation of advanced glycation end products and barrier injury in the ileum intestinal mucosa. These effects were associated to a stronger activation of the lipopolysaccharide-dependent proinflammatory TLR4/NLRP3 inflammasomepathway in the liver of S-Fr mice than of L-Fr mice. In contrast, L-Fr intake induced higher levels of hepatosteatosis andmarkers of fibrosis than S-Fr. Fructoseinduced ex novo lipogenesis with production of SCFA and MCFA was confirmed by metagenomic analysis. These results suggest that consumption of fructose under different forms, liquid or solid, may differently affect gut microbiota, thus leading to impairment in intestinal mucosa integrity and liver homeostasis.

Fructose liquid and solid formulations differently affect gut integrity, microbiota composition and related liver toxicity: a comparative in vivo study

Mastrocola, Raffaella;Ferrocino, Ilario;Liberto, Erica;Chiazza, Fausto;CENTO, ALESSIA SOFIA;COLLOTTA, DEBORA;QUERIO, GIULIA;Nigro, Debora;BITONTO, VALERIA;Cutrin, Juan Carlos;Rantsiou, Kalliopi;Aragno, Manuela;Cordero, Chiara;Cocolin, Luca
Last
;
Collino, Massimo
Co-last
2018-01-01

Abstract

Despite clinical findings suggesting that the form (liquid versus solid) of the sugars may significantly affect the development of metabolic diseases, no experimental data are available on the impact of their formulations on gut microbiota, integrity and hepatic outcomes. In the present sudy, C57Bl/6j mice were fed a standard diet plus water (SD), a standard diet plus 60% fructose syrup (L-Fr) or a 60% fructose solid diet plus water (S-Fr) for 12 weeks. Gut microbiota was characterized through 16S rRNA phylogenetic profiling and shotgun sequencing of microbial genes in ileum content and related volatilome profiling. Fructose feeding led to alterations of the gutmicrobiota depending on the fructose formulation,with increased colonization by Clostridium,Oscillospira and Clostridiales phyla in the S-Fr group and Bacteroides, Lactobacillus, Lachnospiraceae and Dorea in the L-Fr. S-Fr evoked the highest accumulation of advanced glycation end products and barrier injury in the ileum intestinal mucosa. These effects were associated to a stronger activation of the lipopolysaccharide-dependent proinflammatory TLR4/NLRP3 inflammasomepathway in the liver of S-Fr mice than of L-Fr mice. In contrast, L-Fr intake induced higher levels of hepatosteatosis andmarkers of fibrosis than S-Fr. Fructoseinduced ex novo lipogenesis with production of SCFA and MCFA was confirmed by metagenomic analysis. These results suggest that consumption of fructose under different forms, liquid or solid, may differently affect gut microbiota, thus leading to impairment in intestinal mucosa integrity and liver homeostasis.
2018
55
185
199
Fructose; Microbiota; Advanced glycation end products; Fecal volatilome; Inflammasome
Mastrocola, Raffaella; Ferrocino, Ilario; Liberto, Erica; Chiazza, Fausto; Cento, Alessia Sofia; Collotta, Debora; Querio, Giulia; Nigro, Debora; Bitonto, Valeria; Cutrin, Juan Carlos; Rantsiou, Kalliopi; Durante, Mariaconcetta; Masini, Emanuela; Aragno, Manuela; Cordero, Chiara; Cocolin, Luca; Collino, Massimo
File in questo prodotto:
File Dimensione Formato  
JNB, 2018, 185-199.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 4.89 MB
Formato Adobe PDF
4.89 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Post-print_JNB 2018.pdf

Accesso aperto

Tipo di file: POSTPRINT (VERSIONE FINALE DELL’AUTORE)
Dimensione 4.65 MB
Formato Adobe PDF
4.65 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1661849
Citazioni
  • ???jsp.display-item.citation.pmc??? 26
  • Scopus 50
  • ???jsp.display-item.citation.isi??? 48
social impact