Terpenoids are plant metabolites with many biological properties, including a possible antimicrobial activity. Biolog methodology for metabolic characterization of microorganisms has been found suitable for the evaluation of antimicrobial activity of different plant components against bacterial consortia. With such methodology it is possible to ascertain the initial duration of the antimicrobial activity (if present) and its resulting effect on microorganisms’ viability, so allowing a precise screening of different substances vs. different microorganisms. An application of this methodology has been employed for the evaluation of the effect of 6 oxygenate terpenoids at different concentrations that could be normally found in raw milk, against a wide number of lactic acid bacterial strains, isolated from raw milk in previous researches and belonging to the usual physiological and metabolic groups of such microorganisms (thermophilic and mesophilic rods and cocci, homo- and hetero-fermenting). Initial results confirm the suitability of the adopted methodology for screening purposes, indicating that among the different groups tested various types of lactic acid bacteria responses to the presence of the same terpenoid can be detected.
Application of Biolog methology for the evaluation of terpenoids influence on lactic acid bacteria
BELVISO, Simona;AMBROSOLI, Roberto;GIORDANO, Manuela;MINATI, Jose' Luis;ZEPPA, Giuseppe
2011-01-01
Abstract
Terpenoids are plant metabolites with many biological properties, including a possible antimicrobial activity. Biolog methodology for metabolic characterization of microorganisms has been found suitable for the evaluation of antimicrobial activity of different plant components against bacterial consortia. With such methodology it is possible to ascertain the initial duration of the antimicrobial activity (if present) and its resulting effect on microorganisms’ viability, so allowing a precise screening of different substances vs. different microorganisms. An application of this methodology has been employed for the evaluation of the effect of 6 oxygenate terpenoids at different concentrations that could be normally found in raw milk, against a wide number of lactic acid bacterial strains, isolated from raw milk in previous researches and belonging to the usual physiological and metabolic groups of such microorganisms (thermophilic and mesophilic rods and cocci, homo- and hetero-fermenting). Initial results confirm the suitability of the adopted methodology for screening purposes, indicating that among the different groups tested various types of lactic acid bacteria responses to the presence of the same terpenoid can be detected.
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